Journal: Journal of Pharmacy Research

Article Id: JPRS-PHDRS-0000101
Title: Screening of Crude Plant Extracts for anti-adipogenesis activity in 3T3-L1 cells.
Category: Pharmacognosy and Herbal Drugs related study
Section: Research Article
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    Objective: Hyperlipidemia is the major risk factor contributing to prevalence and severity of cardiovascular complications like atherosclerosis. The present study was aimed to screen the selected crude plant extracts for antiadipogenesis activity in-vitro in 3T3-L1 cells. Methodology: Four herbal drugs which were used traditionally in the treatment of several disorders including obesity were selected namely, Commiphora caudata (leaves, bark), Boswellia ovalifoliolata (leaves, gum), Saccharum spontaneum (whole plant) and Garcinia mangostana (fruit pericarp). Ethanolic extracts of the selected plants were screened for their cytotoxicity and anti-adipogenic activity in-vitro. For testing the in-vitro cytotoxicity of the selected plant extracts, an MTT assay was performed with concentrations of test extracts of the selected plants ranging from 31.25 to 1000 mg/mL. Effect of the plant extracts on lipid accumulation in cultured 3T3-L1 adipocytes was determined by measuring Oil Red O staining. The 3T3-L1 adipocytes were cultured and differentiated in a Dulbecco’s Modified Eagle’s Medium containing 10% fetal bovine serum for 6 to 8 days in the absence and presence of plant extracts. Results: Among the six plant extracts tested, ethanolic extract of B.ovalifoliolata gum showed higher cytotoxicity with a lower CTC50 value of 100 µg/ml and the remaining extracts showed CTC50 ranging from 120-720 µg/ml. Adipogenesis was substantially inhibited by the test extracts and among the six extracts screened, C. caudata leaves , B.ovalifoliolata Gum, S. spontaneum whole plant and G. mangostana fruit pericarp significantly reduced the lipid accumulation with 51.5, 46.1, 26 and 56.3 (%) over control at 100, 700, 250 and 250µg/mL respectively through the quantification method of Oil Red O staining. Other two extracts namely C. caudata bark (4.8) and B.ovalifoliolata leaves (8.3)) did not show significant inhibition of fat accumulation at the tested concentration. Conclusion: The results suggest that these extracts may be useful in the treatment of Hyperlipidemia and its associated cardiovascular diseases like atherosclerosis.

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    Author(s) Name:

    Geetha Kodali*1, Sreekanth Kakarla2 and Ganapaty Seru1

    Affiliation(s) Name:

    1Department of Pharmacognosy and Phytochemistry, University College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, Andhra Pradesh, India.
    2Department of Pharmacy, Acharya Nagarjuna University, Guntur, Andhra Pradesh, India.

     

    *Corresponding author.
    Geetha Kodali
    Department of Pharmacognosy and Phytochemistry,
    University College of Pharmaceutical Sciences,
    Andhra University, Visakhapatnam,
    Andhra Pradesh, India.
    Email: geetha_pharma1@yahoo.co.in 

    Received on:21-11-2013; Revised on: 08-12-2013; Accepted on:06-01-2014

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    Author:

    Geetha Kodali*1, Sreekanth Kakarla2 and Ganapaty Seru1

    Title:Screening of Crude Plant Extracts for anti-adipogenesis activity in 3T3-L1 cells.
    Journal:Journal of Pharmacy Research
    Vol(issue):8 (January )
    Year:2014
    Page No: (81-86)
  • Experimental Methods Keywords

    Methodology: cytotoxicity ,anti-adipogenic activity in-vitro,MTT assay
    Research Materials:Commiphora caudata (leaves, bark), Boswellia ovalifoliolata (leaves, gum), Saccharum spontaneum (whole plant) and Garcinia mangostana (fruit pericarp).

Keywords

Adipogenesis Oil red O 3T3 L1 cell line MTT assay Plant extracts

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