Journal: Journal of Pharmacy Research

Article Id: JPRS-PA-0000102
Title: Development and validation of RP-HPLC method for quantitative analysis of Rizatriptan benzoate in pure and Pharmaceutical formulations
Category: Pharmaceutical Analysis
Section: Research Article
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    A reverse phase high performance liquid chromatographic method was performed by using Intersil DBC C18column (250mmX4.6mmX5μ particle size) with UV detection at 225 nm. An isocratic mobile phase consisting of Phosphate buffer pH6.5:Aceto nitrile:Metahnol-87:7.8:5.2 (v/v/v) at a flow rate of 1ml/min.The retention time for Rizatriptan benzoate was 8.435 min. The method was linear in the concentration range of 20-60 μg/ml of Rizatriptan benzoate with the correlation coefficient of 0.999. The method was validated for linearity,accuracy,precision,limit of detection,limit of quantification, robustness and ruggedness.Recovey of Rizatriptan benzoate was found to be101% to 102%.The developed reverse phase high performance liquid chromatographic method was simple, sensitive, precise and accurate and the method was found suitable for estimating in tablet dosage form.

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    Author(s) Name:

    P.Jitendra kumar1, P.Mallikharjuna Rao*2, Desireddy R.B2., D .Sandhaya reddy2, P. Koushik2, V.Sai kishore3

    Affiliation(s) Name:

    1.School of Pharmaceutical Education and Research,Berhampur university,Berhampur 760002,Odisha,India
    2.Nalanda Instituteof Pharmaceutical Sciences, Kantepudi,Satteanpalli 522403, Guntur,AndhraPradesh, India
    3. Bapatla college of Pharmacy,Bapatla 522101 ,Guntur,Andhra Pradesh, India.

    *Corresponding author.
    P.Mallikharjuna rao,
    Nalanda Institute of Pharmaceutical Sciences,
    Kantepudi, Sattenpalli, 522403,
    Guntur, AndhraPradesh, India.

    E-mail: mallikharjun.m.phm@gmail.com



    Received on:17-11-2013; Revised on: 27-12-2013; Accepted on:09-01-2014

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    Author:

    P.Jitendra kumar1, P.Mallikharjuna Rao*2, Desireddy R.B2., D .Sandhaya reddy2, P. Koushik2, V.Sai kishore3

    Title:Development and validation of RP-HPLC method for quantitative analysis of Rizatriptan benzoate in pure and Pharmaceutical formulations
    Journal:Journal of Pharmacy Research
    Vol(issue):8 (January )
    Year:2014
    Page No: (87-90)
  • Experimental Methods Keywords

    Methodology:RP-HPLC method
    Research Materials: Rizatriptan benzoate

Keywords

Rizatriptan benzoate C18 column Reverse phase Validation

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