Journal: Journal of Pharmacy Research

Article Id: JPRS-PA-00001056
Title: Analytical method development and validation of simultaneous determination of Diphenhydramine HCL, Guaiphenesin and Bromhexine HCL in liquid dosage form by RP-HPLC technique
Category: Pharmaceutical Analysis
Section: Research Article
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    The present paper deals with the simultaneous determination of the active ingredients in the multicomponent pharmaceutical product using a RP- HPLC Technique. Diphenhydramine hydrochloride, Guaiphenesin and Bromhexine Hydrochloride were separated and quantitated either in pure form or simultaneously in the other interfering substances such as methyl and propyl parabens which are present in cough syrup. Several trials were carried out to obtain good separation among Diphenhydramine hydrochloride, Guaiphenesin and Bromhexine hydrochloride in mixture. These trials involved the use of different ratios of the mobile phase component, different pH of buffer, different flow rates, and different wavelength. The mobile phase of choice was found to be 0.05M Sodium Acetate in distilled water pH adjusted to 3.0 with acetic acid and solvent (60:40) ratio with the flow rate of 1.3 ml/min on BDS HYPERSIL C18 column with an Internal diameter 250×4.6mm, and 5 micron particle size with UV detection at 258 nm gave a satisfactory Chromatogram with Diphenhydramine Hydrochloride, Bromhexine Hydrochloride and Guaiphenesin of retention time of 10.45min, 16.76 min and 3.5min (Mixed standard) respectively.

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    Author(s) Name:

    Jayalakshmi.B*, Ramesh.J , Kalpana.T.N , Vijayamirtharaj.R.

    Affiliation(s) Name:

    Department of Pharmaceutical Analysis,Jkk Munirajah Medical Research Foundation College of pharmacy,Komarapalayam. 638 183.Namakkal (DT), Tamilnadu
    Received on: 20-08-2010; Revised on: 16-10-2010; Accepted on:10-11-2010

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    Author:

    Jayalakshmi.B*, Ramesh.J , Kalpana.T.N , Vijayamirtharaj.R.

    Title:Analytical method development and validation of simultaneous determination of Diphenhydramine HCL, Guaiphenesin and Bromhexine HCL in liquid dosage form by RP-HPLC technique
    Journal:Journal of Pharmacy Research
    Vol(issue):3 (December)
    Year:2010
    Page No: (2868-2870)
  • Experimental Methods Keywords

    Methodology:RP-HPLC technique
    Research Materials:Diphenhydramine hydrochloride, Guaiphenesin, Bromhexine Hydrochloride

Keywords

Diphenhydramine hydrochloride Guaiphenesin Bromhexine Hydrochloride and RP-HPLC.

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