Journal: Journal of Pharmacy Research

Article Id: JPRS-QA-00001059
Title: Development and validation of a UV spectrophotometric method for estimation of Citicoline sodium in Bulk and Dosage Form
Category: Quality Assurance
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    Development and validation of a simple UV and derivative spectrophotometric method to quantify citicoline sodium used as a single active principle in pharmaceutical dosage forms were done. Pharmacopeias have not yet provided an official method for its quantification. A study was carried out considering all the parameters established by USP XXIV to validate an analytical method i.e. linearity, accuracy, range, precision and specificity. Based on spectrophotometric characteristic of citicoline sodium, a signal at 272nm for simple UV spectrum and at 286nm for derivative spectrum (1D286) was found adequate for quantification. The linearity signal and concentration of citicoline sodium in the range of 10-50µg/ml in aqueous solution presents a correlation coefficient (r2) of 0.999 for simple UV and 0.9997 for 1storder derivative spectrum. The limit of detection (LOD) and limit of quantification (LOQ) was found to be 0.75µg/ml and 2.27µg/ml by simple UV and 0.69µg/ml and 2.09µg/ml by derivative spectrum respectively. The mean recovery percentage was 99.93±1.27% by simple UV and 101.08±0.5% by derivative spectrum. In addition, the proposed method is simple, easy to apply, low-cost, does not use polluting reagent and requires relatively inexpensive instruments.

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    Author(s) Name:

    Jimmi A.Patel*, Bibhuranjan Panigrahi, Chhaganbhai N.Patel, Badmanaban Ramalingan, Kamal Modh

    Affiliation(s) Name:

    *Department of Pharmaceutical Quality Assurance,Shri Sarvajanik Pharmacy College, Near Arvind Baug, Mehsana-384 001, India

    *Corresponding author.
    Jimmi A.Patel
    Department of Pharmaceutical Quality Assurance
    Shri Sarvajanik Pharmacy College,
    Near Arvind Baug, Mehsana-384 001, India


    Received on: 16-07-2010; Revised on: 10-10-2010; Accepted on:19-11-2010

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    Author:

    Jimmi A.Patel*, Bibhuranjan Panigrahi, Chhaganbhai N.Patel, Badmanaban Ramalingan, Kamal Modh

    Title:Development and validation of a UV spectrophotometric method for estimation of Citicoline sodium in Bulk and Dosage Form
    Journal:Journal of Pharmacy Research
    Vol(issue):3(12)
    Year:2010
    Page No: (2876-2878)
  • Experimental Methods Keywords

    Methodology:Development and validation, UV spectrophotometer, Derivative spectrum
    Research Materials:Citicoline sodium

Keywords

Citicoline sodium UV spectrophotometer Derivative spectrum

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