Journal: Journal of Pharmacy Research

Article Id: JPRS-ERS-0000106
Title: Optimization of Polygalacturonase using isolated Bacillus subtilis C4 by submerged fermentation
Category: Enzyme Related Study
Section: Research Article
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    Aim: Pectinases are an enzyme group that catalyzes pectin substance degradation through depolymerization (hydrolases and lyases) and deesterification (esterases) reactions. Method: The present investigation is envisaged to study the production of alkaline and thermostable polygalacturonase using isolated soil Bacillus subtilis(C4) by submerged fermentation. The potential of the isolate was compared with that of procured ones. Effect of fermentation factors like incubation time, temperature, pH, inoculum age, inoculum volume, agitation speed, carbon and nitrogen sources were optimized. Effect of crude substrates like corn, bajra, white jower, yellow jower, wheat bran, lemon peel, lemon pulp, papaya peel, mango peel, apple pomace and orange peel were tested. Result: Process parameters like incubation time (19.65U/mL), temperature (28.08 U/mL), pH 10.0, inoculum volume(1mL), inoculum age (24h) and agitation (150rpm) were studied for maximum production of polygalacturonase. To the optimized medium, different carbon, nitrogen and amino acids supplements were used for the enhancement of enzyme activity. Polygalacturonase production significantly improved when yellow jower was used as a carbon source(42.64U/mL) and casein (45.69 U/mL) as nitrogen sources. Cysteine, glycine, arginine and histidine also stimulated polygalacturonase activity. Conclusion: The present study suggests that the new soil isolate Bacillus subtilis C4 is a good producer of polygalacturonase. Yellow jower as a carbon source significantly influenced enzyme activity. Considering the process advantages of thermostable enzyme and wide applications of alkaline polygalacturonase, the new soil isolate Bacillus subtilis C4 is a potential candidate for commercial exploitations.

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  • About the authors and Affiliations

    Author(s) Name:

    M.P.Kusuma * and D.Sri Rami Reddy

    Affiliation(s) Name:

    Centre for Biotechnology, Faculty of Chemical Engineering and Technology, College of Engineering, Andhra University, Visakhapatnam, Andhra Pradesh, India.

     

    *Corresponding author.
    M.P.Kusuma
    3-2-800, 3rd floor,
    Anand Swaroop Nivas,
    Kachiguda, Hyderabad,A.P.
    Email address: mpkusuma@gmail.com


    Received on:21-11-2013; Revised on: 08-12-2013; Accepted on:12-01-2014

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    Author:

    M.P.Kusuma * and D.Sri Rami Reddy

    Title:Optimization of Polygalacturonase using isolated Bacillus subtilis C4 by submerged fermentation
    Journal:Journal of Pharmacy Research
    Vol(issue):8 (February)
    Year:2014
    Page No: (106-112)
  • Experimental Methods Keywords

    Methodology:Polygalacturonase production by submerged fermentation ,Polygalacturonase assay
    Research Materials:Polygalacturonase

Keywords

Polygalacturonase  Bacillus subtilis yellow jower etc.

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