Journal: Journal of Pharmacy Research

Article Id: JPRS-Tox-0000107
Title: Evaluation of skin corrosive property of ethanolic leaf extract of Acyranthus aspera by in vitro Transcutaneous Electrical Resistance Test and Human skin Model Test.
Category: Toxicology
Section: Research Article
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    Objective: The present article is an attempt to investigate the corrosive property of ethanolic leaf extract of Achyranthus aspera (Amaranthaceae) by in vitro transcutaneous electrical resistance (TER) test and in vitro human skin model test before conducting wound healing experiments on rats to know instead of wound healing activity, whether it is having skin corrosion property or not. Methods: In TER test, application of extract for up to 24 hours to the epidermal surfaces of skin discs in a two-compartment test system in which the skin discs function as the separation between the compartments. Corrosive substances will be identified by their ability to produce a loss of normal stratum corneum integrity and barrier function, which was measured as a reduction in the TER below a threshold level (5 KW). In human skin model test, the extract is applied topically to a three-dimensional human skin model, comprising reconstructed epidermis with a functional stratum corneum for 1 hour. Corrosive substances are identified by their ability to penetrate the stratum corneum by diffusion or erosion, and cytotoxic to the underlying cell layers to produce a decrease in cell viability below threshold levels (15 %). Results: The ethanolic extract of Achyranthus aspera does not show corrosive property in both transcutaneous electrical resistance and human skin model test. Conclusion: The results obtained in these studies suggest a significant scope for the isolation of biological active components for further studies.

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    Author(s) Name:

    Kuntrapakam Hema Kumar1, 2, Dr. Gajanan Rajpal Deshmukh1, Poojari Venkata Suresh Reddy1, 2,Boddapati Srinivasa Rao1, Suryadevara Surya Narayana1, Chirumamilla Venkata Satish Kumar1 and Dr. Lokanatha Valluru†*.

    Affiliation(s) Name:

    1 Scientist, Toxicology, Aptus Biosciences Private Limited, Mahabubnagar – 509002, India.
    2 Research scholar, Department of Biotechnology, Dravidian University, Kuppam – 517425, India.
    Assistant professor, Department of Biotechnology, Dravidian University, Kuppam – 517425, India.


    *Corresponding author.
    Dr. Lokanatha Valluru
    Department of Biotechnology,
    School of Herbal Studies & Naturo Sciences,
    Dravidian University,
    Kuppam – 517 426,
    Andhra Pradesh, India.
    E-mail : lokanathav@yahoo.co.in


    Received on:26-11-2013; Revised on: 19-12-2013; Accepted on:12-01-2014

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    Author:

    Kuntrapakam Hema Kumar1, 2, Dr. Gajanan Rajpal Deshmukh1, Poojari Venkata Suresh Reddy1, 2,Boddapati Srinivasa Rao1, Suryadevara Surya Narayana1, Chirumamilla Venkata Satish Kumar1 and Dr. Lokanatha Valluru†*.

    Title:Evaluation of skin corrosive property of ethanolic leaf extract of Acyranthus aspera by in vitro Transcutaneous Electrical Resistance Test and Human skin Model Test.
    Journal:Journal of Pharmacy Research
    Vol(issue):8 (February)
    Year:2014
    Page No: (113-117)
  • Experimental Methods Keywords

    Methodology:Electrical Resistance Test and Human skin Model Test
    Research Materials:Acyranthus aspera

Keywords

Epidermis EpiSkin MTT Stratum corneum Transcutaneous electrical resistance.

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