Journal: Journal of Pharmacy Research

Article Id: JPRS-PA-00001074
Title: Development and validation of spectrophotometric and HPLC methods For the determination of Famotidine in bulk drug formulations.
Category: Pharmaceutical Analysis
Section: Research Article
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    Two simple, rapid and selective methods were developed for the determination of Famotidine in drug substance and its pharmaceutical preparations. The first method (I) is based on UV Spectroscopy.UV spectra of Famotidine are recorded. The absorption maxima (lmax) were observed at 286nm. Beer’s law was obeyed over the concentration range from 2 to 20 µg/ml and it shows linearity The validation of the proposed method was further confirmed by Recovery studies at 50%, 100% and 150%. The percentage recovery values from 96.83%w/w, 102.7%w/w, 97.70%w/w, 99.07%w/w. This serves as a good index of accuracy and reproducibility of the study. The second method is based on the Reverse phase HPLC method was developed by using Methanol: Acetonitrile in the ratio of 50:50 as a mobile phase and Hypersil C18 (5 micron, 250 4.6 mm) column as a stationary phase. Flow rate was 1.5 ml/min. The uv detector was operated at 286nm. The method was validated for specificity, linearity, precision, accuracy and limit of quantification. The recovery studies showed that the observed percentage recovery of Famotidine was found to be 98.4 to 98.9 %. The retention time of Famotidine was found to be 5.907min. The developed method was accurate and precise which was evident from the analytical data and recovery studies.

    Cite this article as:M.V. Kumudhavalli, S.Alexandar, and B. Jaykar,Development and validation of spectrophotometric and HPLC methods For the determination of Famotidine in bulk drug formulations,Journal of Pharmacy Research 2015,9(8),562-568

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    Author(s) Name:

    M.V. Kumudhavalli*, S.Alexandar, and B. Jaykar

    Affiliation(s) Name:

    Department of Pharmaceutical Analysis,Vinayaka Mission’s College of Pharmacy,
    Yercaud main road, Kondappanaikenpatty, Salem-636008, Tamilnadu ,India

    *Corresponding author.
    Dr.M.V.Kumudha valli,M.Pharm,Ph.D
    Department of Pharmaceutical Analysis,
    Vinayaka Mission’s College of Pharmacy,
    Yercaud main road, Kondappanaikenpatty,
    Salem-636008, Tamilnadu ,India


    Received on:24-06-2015; Revised on: 28-07-2015; Accepted on: 25-08-2015

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    Author:

    M.V. Kumudhavalli*, S.Alexandar, and B. Jaykar

    Title:Development and validation of spectrophotometric and HPLC methods For the determination of Famotidine in bulk drug formulations.
    Journal:Journal of Pharmacy Research
    Vol(issue):9 (August)
    Year:2015
    Page No: (562-568)
  • Experimental Methods Keywords

    Methodology: UV- Spectroscopy, Reverse-phase HPLC
    Research Materials:Famotidine

Keywords

Famotidine; UV- Spectroscopy Reverse-phase HPLC.

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