Journal: Inter. J. of Chemical and Analytical Science

Article Id: JPRS-AC-00001098
Title: Method development and Validation of Reverse Phase HPLC Method for the Determination of Dithranol in Pharmaceutical Ointment Forms
Category: Analytical Chemistry
Section: Research Article
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    A simple and rapid reverse phase high performance liquid chromatography (RP-HPLC) method was developed and validated for quantitative determination of dithranol in Ointments. Dithranol was analyzed by using reverse phase Phenomenex C18 column (4.6mm x 25cm, 5microns) with mobile phase consisting of acetonitrile: water: TFA (60:40:0.05%v/v). The flow rate was set 1.2ml/min and the analysis was performed at wavelength 254nm using Photo Diode Array (PDA) detector at 25°C temperature. The method was validated and stability studies were conducted under different conditions. The retention time for dithranol was around 10.2minutes. The developed method was successfully applied to estimate the amount of dithranol in ointment formulations and it is the stability indicating method.

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    Author(s) Name:

    D Chakravarthy, K Prasanth Kumar, AU Srikanth, Bulusu Bhanuteja, D Peer Basha*

    Affiliation(s) Name:

    TherDose Pharma Pvt. Ltd, Plot No. 118-120, Road No.5, ALEAP Ind. Est., Pragathi Nagar, Opp. JNTU, Hyderabad, Andhra Pradesh – 500072.

    Corresponding Author:
    D Peer basha, TherDose Pharma Pvt. Ltd, Plot No. 118-120, Road No.5, ALEAP Ind. Est., Pragathi Nagar, Hyderabad– 500072., Andhra
    Pradesh
    Received 23-02-2011; Revised 13-04-2011; Accepted 03-05-2011

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    Author:

    D Chakravarthy, K Prasanth Kumar, AU Srikanth, Bulusu Bhanuteja, D Peer Basha*

    Title:Method development and Validation of Reverse Phase HPLC Method for the Determination of Dithranol in Pharmaceutical Ointment Forms
    Journal:Inter. J. of Chemical and Analytical Science
    Vol(issue):2 (July)
    Year:2011
    Page No: (73-78)
  • Experimental Methods Keywords

    Methodology:Method development and Validation of Reverse Phase HPLC Method
    Research Materials:Dithranol in Pharmaceutical Ointment Forms

Keywords

RP-HPLC; PDA; Dithranol

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