Journal: Journal of Pharmacy Research

Article Id: JPRS-PB-00001105
Title: Assortment of vital nutrients for enhancement of Cyclodextrin glycosyltransferase produced by Bacillus amyloliquefaciens KST5: A Plackett-Burman design approach
Category: Pharmaceutical Biotechnology
Section: Research Article
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    Background: Cyclodextrin glycosyltransferase (CGTase, EC 2.4.1.19) is the enzyme which have the capability to convert various sugars and starch into cyclodextrins (CDs). These CDs enables their broad application in the pharmaceutical, food, cosmetics, agriculture, analytical chemistry and biotechnology industries. Methods: In the present study various carbon and nitrogen sources were screened for enhanced CGTase production by Bacillus amyloliquefaciens KST5. A Plackett-Burman design (PBD) was employed to screen the 15 nutrients. All compounds were studied at 3 levels (-1, 0, +1). Results and discussion: Results indicates that among selected 15 nutrients yeast extract, peptone and starch showed highest impact on the CGTase production. Between 15 studied nutrients 5 compounds such as potassium nitrate, fructose, urea, dextrose and lactose were found to be insignificant for CGTase production. It was observed that yeast extract and starch at lower concentrations are favorable for hyper CGTase production however peptone is needed at higher concentrations. Conclusion: With the help of PB design important nutrients which have the highest influence on the CGTase production were screened. This study implies that the PBD is an efficient tool to screen the large number of compounds with less number of runs.

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    Author(s) Name:

    Abhishek Sivaramareddya, Polarapu sriharia, Devarapalli Keziaa,b*.

    Affiliation(s) Name:

    aDepartment of Biotechnology, St. Martin’s Engineering College, Secunderabad, India.
    b Current address: Andaman and Nicobar Centre for Ocean Science and Technology,
    ESSO-National Institute of Ocean Technology, Port Blair 744103, India.

    *Corresponding author.
    Dr. D. Kezia
    Department of Biotechnology,
    St. Martin’s Engineering College,
    Secunderabad, India.


    Received on:02-07-2014; Revised on: 17-08-2014; Accepted on:03-09-2014

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    Author:

    Abhishek Sivaramareddya, Polarapu sriharia, Devarapalli Keziaa,b*.

    Title:Assortment of vital nutrients for enhancement of Cyclodextrin glycosyltransferase produced by Bacillus amyloliquefaciens KST5: A Plackett-Burman design approach
    Journal:Journal of Pharmacy Research
    Vol(issue):8 (September)
    Year:2014
    Page No: (1191-1197)
  • Experimental Methods Keywords

    Methodology:Placket-Burmen Design, Optimization, Submerged fermentation, Factorial design, Screening Designs.
    Research Materials:Cyclodextrin glycosyltransferase

Keywords

CGTase Placket-Burmen Design Optimization Submerged fermentation Factorial design Screening Designs.

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