Journal: Journal of Pharmacy Research

Article Id: JPRS-MB-0000112
Title: Evaluation of antimicrobial activity and radical scavenging potential of lipopeptide biosurfactant from Klebsiella pneumoniae MSO-32
Category: Microbiology
Section: Research Article
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    Objective: In the present study we evaluated the antimicrobial and antiradical potential of a lipopeptide biosurfactant isolated from a soil bacterium, Klebsiella pneumoniae MSO-32 strain. Methods: Biosurfactant was produced by shake flask fermentation and purified by column chromatography. Its antimicrobial activity was estimated against gram-positive and gram-negative bacteria using agar well diffusion method. MIC and MBC values were determined to find efficacy of biosurfactant against test bacteria. The effect of biosurfactant on cell wall integrity of sensitive strain M. luteus was determined in terms of leakage of UV absorbing intracellular components. Antiradical potential and metal chelation capacity of biosurfactant was determined by using DPPH and hydroxyl radical scavenging assay and iron chelation activity respectively. Results: Chemically, biosurfactant was lipopeptide in nature and exhibited notable antimicrobial activity against all test bacteria. M. luteus displayed highest sensitivity with MIC at 10µg/ml and MBC at 30µg/ml. The biosurfactant caused gradual leakage of proteins, DNA and RNA from treated cells and showed concentration dependent scavenging effect on DPPH, hydroxyl radical and ferrous ion. Conclusion: The biosurfactant of K. pneumoniae MSO-32 exhibited promising antimicrobial and antiradical potential.

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    Author(s) Name:

    H. J. Bhosale*., T. A. Kadam., S. Phulari

    Affiliation(s) Name:

    School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded (M.S)-431606, India.

     

    *Corresponding author.
    H. J. Bhosale
    School of Life Sciences,
    Swami Ramanand Teerth Marathwada University,
    Nanded (M.S),-431606, India.
    E-mail : bhoslehemlata@gmail.com


    Received on:15-12-2013; Revised on: 08-01-2014; Accepted on:20-01-2014

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    Author:

    H. J. Bhosale*., T. A. Kadam., S. Phulari

    Title:Evaluation of antimicrobial activity and radical scavenging potential of lipopeptide biosurfactant from Klebsiella pneumoniae MSO-32
    Journal:Journal of Pharmacy Research
    Vol(issue):8 (February)
    Year:2014
    Page No: (139-143)
  • Experimental Methods Keywords

    Methodology:shake flask fermentation
    Research Materials:lipopeptide biosurfactant

Keywords

Oxidative stress surface active molecules antimicrobial action antioxidant potential lipopeptide biosurfactants K. pneumoniae

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