Journal: Journal of Pharmacy Research

Article Id: JPRS-PA-00001135
Title: Sensitive liquid chromatography tandem mass spectrometry method for the quantification of Ropinirole in human plasma using liquid-liquid extraction technique
Category: Pharmaceutical Analysis
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    Background: A sensitive high-performance liquid chromatography – positive ion electroscopy tandem mass spectrometry method was developed and validated for the quantification of Ropinirole in human plasma. Methods: Following liquid-liquid extraction, the analyte and internal standard were separated using an isocratic mobile phase on a reverse phase C18 column and analyzed by MS/MS in the multiple reaction monitoring mode using the respective [M+H]+ ions, m/z is 261 to 114 for Ropinirole and m/z is 325 to 262 for the internal standard. Results and discussion: The assay exhibited a linear dynamic range of 10.0 pg/mL to 1006.3 pg/mL for Ropinirole in human plasma. The lower limit of quantification was 10.0 pg/mL with a relative standard deviation of less than 7%. This LC-MS/MS method was validated with between-batch and within-batch precision of 5.5 to 8.6% and 4.3 to 10.9%, respectively. The between-batch and within-batch accuracy was 99.0 to 104.4% and 94.1 to 107.4%, respectively. Conclusion: This validated method is simple, rapid, cost effective and repeatable enough to be used in pharmacokinetic studies.

    Cite this article as: Sreekanth Kakarla1* and Ganapaty Seru1,Sensitive liquid chromatography tandem mass spectrometry method for the quantification of Ropinirole in human plasma using liquid-liquid extraction technique, Journal of Pharmacy Research 2015,9(12),662-669.

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    Author(s) Name:

    Sreekanth Kakarla1* and Ganapaty Seru 2

    Affiliation(s) Name:

    1 Department of Pharmacy, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur, Andhra Pradesh, India.
    2 Gitam Institute of Pharmacy, Gitam University, Visakhapatnam, Andhra Pradesh, India.

    *Corresponding author.
    Sreekanth Kakarla
    Department of Pharmacy,
    Acharya Nagarjuna University,
    Nagarjuna Nagar, Guntur,
    Andhra Pradesh, India.

    Received on:06-10-2015; Revised on: 17-10-2015; Accepted on: 20-12-2015

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    Author:

    Sreekanth Kakarla1* and Ganapaty Seru 2

    Title:Sensitive liquid chromatography tandem mass spectrometry method for the quantification of Ropinirole in human plasma using liquid-liquid extraction technique
    Journal:Journal of Pharmacy Research
    Vol(issue):9 (December)
    Year:2015
    Page No: (662-669)
  • Experimental Methods Keywords

    Methodology:Liquid Chromatography-Tandem Mass Spectrometry
    Research Materials:Ropinirole

Keywords

Ropinirole liquid chromatography-tandem mass spectrometry human plasma isocratic and pharmacokinetic studies.

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