Journal: Inter. J. of Chemical and Analytical Science

Article Id: JPRS-AC-00001152
Title: Stability- Indicating High Performance Thin Layer Chromatography Determination of Tenofovir Disoproxil Fumarate in Bulk and Tablet Dosage Form
Category: Analytical Chemistry
Section: Research Article
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    Stability-indicating high-performance thin-layer chromatographic method of tenofovir disoproxil fumarate both as a bulk drug and in formulation was developed and validated as per the International Conference on Harmonization guidelines .The method employed TLC aluminium plates precoated with silica gel 60F-254 as the stationary phase. The solvent system consisted of butanol: ethyl acetate: acetic acid (4.5:0.5:0.3 v/v) and densitometric analysis was carried out in the absorbance mode at 260 nm. The system was found to give compact spot for tenofovir disoproxil fumarate (Rf value of 0.51 ± 0.02). The linear regression analysis data for the calibration plots showed good relationship with r2= 0.9999 ± 0.0001 in the concentration range 300 – 1800 ng/spot. Tenofovir disoproxil fumarate was subjected to acid and alkali hydrolysis, oxidation, thermal degradation and photo degradation. There was no interference of the excipients on the determination of the active pharmaceutical ingredients. Proposed method is precise, selective and accurate for the estimation of tenofovir disoproxil fumarate in the bulk and dosage form.

    Cite This article as: Charushila H. Bhirud *, Dr.S.N.Hiremath,Stability- Indicating High Performance Thin Layer Chromatography Determination of Tenofovir Disoproxil Fumarate in Bulk and Tablet Dosage Form,International Journal of Chemical and Analytical Science 2014,5(1),1-5.

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    Author(s) Name:

    Charushila H. Bhirud *1, Dr.S.N.Hiremath2

    Affiliation(s) Name:

    1Ph.D. Scholar, Pravara Institute of Medical Sciences Deemed University (PIMS),Loni, Maharashtra, India.
    2Principal, Pravara Rural College of Pharmacy for Women, Chincholi, Nasik, Pin.422102, Maharashtra, India.

    *Corresponding author.
    Charushila H. Bhirud
    Ph.D. Scholar,
    Pravara Institute of Medical Sciences
    Deemed University (PIMS),
    Loni, Maharashtra, India.

    Received on: 17-11-2013; Revised on: 29-11-2013; Accepted on:15-12-2013

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    Author:

    Charushila H. Bhirud *1, Dr.S.N.Hiremath2

    Title:Stability- Indicating High Performance Thin Layer Chromatography Determination of Tenofovir Disoproxil Fumarate in Bulk and Tablet Dosage Form
    Journal:Inter. J. of Chemical and Analytical Science
    Vol(issue):5 (January)
    Year:2014
    Page No: (1-5)
  • Experimental Methods Keywords

    Methodology:HPTLC; Validation; Stability-indicating; Degradation
    Research Materials:Tenofovir disoproxil fumarate

Keywords

Tenofovir disoproxil fumarate; HPTLC; Validation; Stability-indicating; Degradation

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