Journal: Inter. J. of Chemical and Analytical Science

Article Id: JPRS-AC-00001156
Title: Fabrication of Nafion/HRP-SWCNT/MWCNT-ZnO based alcohol biosensor: Diagnostics for forensic analysis
Category: Analytical Chemistry
Section: Research Article
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    Nafion/HRP-SWCNT/MWCNT-ZnO working electrode was fabricated. The principle enzyme alcohol oxidase was immobilized on inner surface of PVC beaker which forms the body of electrochemical cell. Optimization of the sensor system was performed and, for electrochemical measurements, a potential (E) of -50 mV versus Ag/AgCl reference electrode was chosen in 0.1M sodium phosphate buffer at pH 8.0. The ethanol content in standard solutions was determined and the biosensor exhibit a linear range from 10 µM to 750 µM with detection limit of 10 µM and a response time of about 10s. These characteristics allowed its application for detection of alcohol concentration in the saliva and blood samples. Therefore, the present study demonstrated that immobilized alcohol oxidase has high specificity toward ethanol and showed good storage stability, which may be of considerable interest for clinical and forensic application.

    Cite this article as:Anjum Gahlaut, Monika Dahiya,Rekha Dhull,Neeraj Dilbagi, Fabrication of Nafion/HRP-SWCNT/MWCNT-ZnO based alcohol biosensor:Diagnostics for forensic analysis,International Journal of Chemical and Analytical Science 2014,5(1),15-20.

     

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    Author(s) Name:

     

    *1Anjum Gahlaut, 1Monika Dahiya, 2Rekha Dhull, 3Neeraj Dilbagi

    Affiliation(s) Name:

    1Centre for Biotechnology, Maharshi Dayanand University, Rohtak,-124001, India
    2Department of Biochemistry, Maharishi Dayanand University, Rohtak, - 124001, India
    3Department of Bio & Nanotechnology, Guru Jambeshwar University of Science & technology, Hisar-125001, India

     

    *Corresponding author.
    Dr. Anjum Gahlaut
    Centre for Biotechnology
    Maharshi Dayanand University,
    Rohtak, - 124001, India


    Received on:20-11-2013; Revised on: 30-11-2013; Accepted on:20-12-2013

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    Author:

     

    *1Anjum Gahlaut, 1Monika Dahiya, 2Rekha Dhull, 3Neeraj Dilbagi

    Title: Fabrication of Nafion/HRP-SWCNT/MWCNT-ZnO based alcohol biosensor: Diagnostics for forensic analysis
    Journal:Inter. J. of Chemical and Analytical Science
    Vol(issue):5 (January)
    Year:2014
    Page No: (15-20)
  • Experimental Methods Keywords

    Methodology:Alcohol oxidase, HRP, Forensics.
    Research Materials:alcohol biosensor

Keywords

Alcohol Biosensor Alcohol oxidase HRP Forensics.

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