Journal: Inter. J. of Chemical and Analytical Science

Article Id: JPRS-AC-00001163
Title: Stability-indicating HPTLC determination of mefenamic acid in bulk drug and pharmaceutical formulations.
Category: Analytical Chemistry
Section: Research Article
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    High-performance thin-layer chromatographic method was described for the analysis of Mefenamic acid in powder and in pharmaceutical formulations. The method employed HPTLC aluminum plates precoated with silica gel 60F254 as the stationary phase and mixture of petroleum ether–chloroform–glacial acetic acid (5.0 : 3.0 : 0.5, v/v/v) as mobile phase. This system gave compact spots for Mefenamic acid with Rf value of 0.60. Densitometric analysis of mefenamic acid was carried out in the absorbance mode at 283 nm. The linear concentration range was 0.2–3.0 µg per spot with correlation coefficient of 0.9987. The limits of detection and quantitation were 0.09 and 0.30 µg per spot, respectively. Mefenamic acid was subjected to acid and alkali hydrolysis, oxidation and photodegradation. The degraded product was well separated from the parent drug. The drug does not undergo degradation under photo, oxidative and acidic conditions. Degradation with sodium hydroxide showed additional spot at Rf value of 0.32. This indicates that the drug is susceptible to alkaline degradation. Statistical analysis proves that the method is repeatable and selective for the estimation of this drug. As the method could effectively separate the drug from its degradation product, it considered as a stability-indicating one. Furthermore, the method was successfully applied for mefenamic acid analysis in commercially available tablets.

    Cite this article as: Safaa F. Saleh, Sayed M. Dereaya  and Mahmoud A.Omar,Stability-indicating HPTLC determination of mefenamic acid in bulk drug and pharmaceutical formulations,International Journal of Chemical and Analytical Science 2014,5(1),55-60.

     

     

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    Author(s) Name:

    Safaa F. Saleh a, b, Sayed M. Dereaya a and Mahmoud A.Omar a

    Affiliation(s) Name:

    a Analytical Chemistry Department, Faculty of Pharmacy, Minia University, Minia, Egypt
    b Pharmaceutical chemistry department, Faculty of pharmacy, Jizan University, Kingdom of Saudia Arabia

    *Corresponding author.
    Safaa F. Saleh
    Analytical Chemistry Department,
    Faculty of Pharmacy,
    Minia University, Minia, Egypt


    Received on: 17-12-2013; Revised on: 29-12-2013; Accepted on:15-01-2014

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    Author:

    Safaa F. Saleh a, b, Sayed M. Dereaya a and Mahmoud A.Omar a

    Title:Stability-indicating HPTLC determination of mefenamic acid in bulk drug and pharmaceutical formulations.
    Journal:Inter. J. of Chemical and Analytical Science
    Vol(issue):5 (January)
    Year:2014
    Page No: (55-60)
  • Experimental Methods Keywords

    Methodology:HPTLC; Stability indicating; Degradation
    Research Materials:Mefenamic acid

Keywords

Mefenamic acid; HPTLC; Stability indicating; Degradation

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