Journal: Inter. J. of Chemical and Analytical Science

Article Id: JPRS-AC-00001167
Title: Simultaneous determination of Lansoprazole and Metronidazole in Pharmaceutical Dosage Form by Reversed Phase- High Performance liquid Chromatography
Category: Analytical Chemistry
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    A simple, selective, rapid, precise and economical reverse phase HPLC method has been developed for the simultaneous estimation of Lansoprazole and Metronidazole in Pharmaceutical dosage forms. The method was carried out on a C18 (25 cm×4.6 mm i.d, 5 µm particle size) column with a mobile phase consisting of Acetonitrile ,water and Triethanol amine(pH 7.3 by addition of O-phosphoric acid) (40:60:1 v/v) at a flow rate of 1 ml/min. Detection was carried out at 290nm with UV detector. The retention time of Metronidazole and Lansoprazole was 3.14 and 10.13 min, respectively. The developed method was validated in terms of accuracy, precision, linearity, limit of detection and limit of quantitation .The calibration curves were linear with R2 between 0.99to 1.0 over a concentration range of 5-25 µg/ml for Metronidazole and 2-10 µg/ml for Lansoprazole . The RSD for intraday and interday precision was <1.0%.The proposed method can be used for the estimation of these drugs in combined dosage forms. Degradation studies showed that solutions of both the drug were stable for 5 hr at room temperature but both the drug showed degradation when subjected to thermal and photodegradation.

    Cite this article as: Snehal Y. Kane, Snehalatha B., Munira Momin,Simultaneous determination of Lansoprazole and Metronidazole in Pharmaceutical Dosage Form by Reversed Phase- High Performance liquid Chromatography,International Journal of Chemical and Analytical Science 2014,5(2),80-85

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    Author(s) Name:

    Snehal Y. Kane1, Snehalatha B.2, Munira Momin2.

    Affiliation(s) Name:

    1 R K University, Rajkot, Gujarat. India
    2 Oriental College of pharmacy, Sanpada, Navi Mumbai. 400705. India

    *Corresponding author.
    Snehal Y Kane.
    Oriental College of pharmacy,
    Sect 2. Sanpada,
    Navi Mumbai-400705,India.


    Received on: 28-06-2014; Revised on: 06-08-2013; Accepted on:17-09-2014

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    Author:

    Snehal Y. Kane1, Snehalatha B.2, Munira Momin2.

    Title:Simultaneous determination of Lansoprazole and Metronidazole in Pharmaceutical Dosage Form by Reversed Phase- High Performance liquid Chromatography
    Journal:Inter. J. of Chemical and Analytical Science
    Vol(issue):6 (January)
    Year:2015
    Page No: (80-85)
  • Experimental Methods Keywords

    Methodology:RP-HPLC
    Research Materials:Lansoprazole, Metronidazole

Keywords

Lansoprazole Metronidazole RP-HPLC.

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