Journal: Journal of Pharmacy Research

Article Id: JPRS-PCS-00001219
Title: Development and validation of RP-HPLC method for simultaneous estimation of atrovastatin and olmesartan in pharmaceutical formulations
Category: Pharmaceutics
Section: Research Article
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    Combination therapy of Atorvastatin (AT) and Olmesartan (OLM) is used for the treatment of co existing essential hypertension and hyperlipidemia in adult persons. In the present study a simple, precise, rapid, efficient and reproducible reversed phase high performance liquid chromatography (RP-HPLC) method has been developed for the simultaneous estimation of AT and OLM present in its tablet dosage forms. Chromatographic separations were carried out isocratically at 30°C ± 0.5°C on a hypersil bds C18 (100,4.6 mm,5µ) with a mobile phase composed of Sodium dihydrogen phosphate pH 4.5 with dilute phosphoric acid : Methanol: Acetonitrile in the ratio of 65:20:15 % v/v at a flow rate of 1.0 ml/min. Detection is carried out using a UV detector at 232 nm. The retention times for OLM and AT were 2.23 min and 3.46 min respectively. The linearity range for 20-100 ìg/ml AT and OLM were found to be 40-200 ìg/ml with correlation coefficient of 0.999 and 0.999 respectively. The % recovery of the proposed method was found in the range of 100.39 for AT and 98.53 for OLM. The relative standard deviations for three replicate measurements in three concentrations of standard solution were always less than 2%. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which may be useful for the routine estimation of AT and OM in bulk drug and in its pharmaceutical dosage form.

    Cite this article as: P.Koushik koteswara rao, D.Pavan kumar reddy,Dr.Brahma reddy,P.Jitendra kumar, Development and validation of RP-HPLC method for simultaneous estimation of atrovastatin and olmesartan in pharmaceutical formulations,Journal of Pharmacy Research 2016,10(2),116-119.

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    Author(s) Name:

    P . Koushik koteswara rao*1 , D.Pavan kumar reddy2, Dr.Brahma reddy3, P.Jitendra kumar4

    Affiliation(s) Name:

     

    1,2,3 Nalanda Institute of Pharamaceutical sciences,kantepudi ,Sattenaplli,Gntur,Andhra Pradesh,India
    4School of Pharmaceutical Education and Research,Berhampuruniversity,Brahampur 760007 ,Odisha, India.

    *Corresponding author.
    P .Koushik koteswara rao
    Nalanda Institute of Pharmaceutical Sciences,
    Kantepudi, Sattenpalli-522403, Guntur,
    AndhraPradesh, India

    Received on:26-12-2015; Revised on: 17-01-2015; Accepted on: 28-02-2016

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    Author:

    P . Koushik koteswara rao*1 , D.Pavan kumar reddy2, Dr.Brahma reddy3, P.Jitendra kumar4

    Title:Development and validation of RP-HPLC method for simultaneous estimation of atrovastatin and olmesartan in pharmaceutical formulations
    Journal:Journal of Pharmacy Research
    Vol(issue):10 (February)
    Year:2016
    Page No: (116-119)
  • Experimental Methods Keywords

    Methodology:RP-HPLC,Validation
    Research Materials:Atorvastatin, Olmesartan

Keywords

Atorvastatin Olmesartan RP-HPLC Validation

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