Journal: Journal of Pharmacy Research

Article Id: JPRS-BMB-00001234
Title: Local tissue damage induced by Echis carinatus venom: Neutralization by Albizia lebbeck seed aqueous extract in mice model
Category: Biochemistry and Molecular Biology
Section: Research Article
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    Background: Albizia lebbeck L. (Fabaceae) is an Indian traditional medicinal plant used in treatment of snakebite and scorpion sting. The present study aims to determine its scientific basis. Methods: Pulverized seed aqueous extracts (cold and hot) was evaluated for neutralization of enzyme activities (hyaluronidases, proteases and PLA2s) of Echis carinatus S. (Viperidae) venom (ECV), in vitro and local tissue damage using murine model. Results and discussion: Cold extract contained proteins which resolved into three fractions on Sephadex G-50 (ALF-1, ALF-2, and ALF-3), while hot extract (ALHE) contained no proteins. ALF-1 contained proteins and did not inhibit any enzymes. ALF-2 and ALHE contained flavonoids, phenolics, saponins, and steroids. All four fractions were positive for peptides/amino acids. ALF-2 and ALHE demonstrated significant inhibition of hyaluronidase. Only ALHE showed potent inhibition of proteases, ECV induced hemorrhage, myotoxicity and pro-coagulant activity. ALF-3 inhibited PLA2 activity and edema. In summary, hyaluronidases and proteases inhibitory phytochemicals present in A. lebbeck seed get extracted in both cold and hot water. The PLA2s inhibiting peptides/amino acids get extracted largely in cold water. Therefore, this differential extraction method can be used to isolate specific inhibitors. Conclusion: The results support the significance of A. lebbeck extracts towards management of snakebite by the local traditional healers in India.

    Cite this article as :  P U Amog, M Yariswamy, Vikram Joshi, A N Nanjaraj Urs, K N Suvilesh, V N Manjuprasanna, M N Savitha, G V Rudresha, Nataraju Angaswamy, B S Vishwanath,T Veerabasappa Gowda,Local tissue damage induced by Echis carinatus venom: Neutralization by Albizia lebbeck seed aqueous extract in mice model,Journal of Pharmacy Research 2016,10(4),167-175.

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    Author(s) Name:

    P U Amog1, M Yariswamy2, Vikram Joshi1, A N Nanjaraj Urs1, K N Suvilesh1, V N Manjuprasanna1, M N Savitha1, G V Rudresha1, Nataraju Angaswamy3, B S Vishwanath1* and T Veerabasappa Gowda1

    Affiliation(s) Name:

    1Department of Studies in Biochemistry, University of Mysore, Manasagangothri, Mysuru, Karnataka, India
    2Department of Medicine, Missouri University, Columbia, MO, 65201, USA
    3Department of Biochemistry, Karnataka State Open University, Mysuru, Karnataka, India

    *Corresponding author.
    Prof. B S Vishwanath, Ph.D
    Department of Studies in Biochemistry
    University of Mysore, Manasagangothri
    Mysuru - 570 006, Karnataka, India.


    Received on:16-01-2016; Revised on: 23-02-2016; Accepted on: 29-03-2016

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    Author:

    P U Amog1, M Yariswamy2, Vikram Joshi1, A N Nanjaraj Urs1, K N Suvilesh1, V N Manjuprasanna1, M N Savitha1, G V Rudresha1, Nataraju Angaswamy3, B S Vishwanath1* and T Veerabasappa Gowda1

    Title:Local tissue damage induced by Echis carinatus venom: Neutralization by Albizia lebbeck seed aqueous extract in mice model
    Journal:Journal of Pharmacy Research
    Vol(issue):10 (April)
    Year:2016
    Page No: (167-175)
  • Experimental Methods Keywords

    Methodology:ECV hyaluronidase, ECV protease, ECV PLA2
    Research Materials:Albizia lebbeck L.

Keywords

ECV hyaluronidase ECV protease ECV PLA2 Folk medicine Complementary medicine Hemorrhage Myotoxicity Edema

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