Journal: Journal of Pharmacy Research

Article Id: JPRS-MB-00001284
Title: Antibacterial activity of silver nanoparticles against methicillin-resistant Staphylococcus aureus synthesized using model Streptomyces sp. pigment by photo-irradiation method
Category: Microbiology
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    Background: In the past few years, the rates of morbidity and mortality are increasing due to methicillin-resistant Staphylococcus aureus (MRSA) infection so, studies in controlling this infection is gravely required. Silver nanoparticles antimicrobial activity were well known but rapid and green synthesis of silver nanoparticles having stupendous antimicrobial activity against MSRA is needed. Aim: Synthesis and evaluation of antibacterial activity of silver nanoparticles against MRSA. Methods: Thepigment produced by Streptomyces coelicolorwasused to reduceAgNO3 in solution to yield silver nanoparticles using photo-irradiation. The synthesized silver nanoparticles were characterized by UVevisible spectroscopy; X-ray diffraction (XRD), transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR). The synthesized silver nanoparticles were tested for antibacterial activity against MRSA, and the synergetic effects, of the combination of silver nanoparticles and antibiotics were evaluated. Results: We developed rapid synthesis for silver nanoparticles using S. coelicolor pigment by photo-irradiation within 20 min. The UVevisible spectroscopy result show maximum absorption between 400 and 450 nm a preliminary confirmation for nanoparticles synthesis. The XRD result confirms the crystalline nature of silver nanoparticles. The TEM image shows the particles are irregular having the size in a range of 28e50 nm. The FTIR result indicates the pigment as the probable reducing agent. The silver nanoparticles alone and in combination with antibiotics, exhibited antibacterial activity against MRSA. Conclusions: The rapid synthesis method developed in this study for the synthesis of silver nanoparticles has distinct advantages over traditional synthesis as the process is less time consuming. The kinetics of silver nanoparticles synthesis using S. coelicolor pigment with photo-irradiation indicates an appropriate way to develop green technology for the bulk synthesis. Furthermore, these biosynthesized silver nanoparticles, alone and in combination with antibiotics, exhibited excellent antimicrobial activity against a MRSA which could be an alternate drug of choice.

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    Author(s) Name:

    Deene Manikprabhu, K. Lingappa*

    Affiliation(s) Name:

    Department of Microbiology, Gulbarga University, Gulbarga 585106, Karnataka, India

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    Author:

    Deene Manikprabhu, K. Lingappa*

    Title:Antibacterial activity of silver nanoparticles against methicillin-resistant Staphylococcus aureus synthesized using model Streptomyces sp. pigment by photo-irradiation method
    Journal:Journal of Pharmacy Research
    Vol(issue):6 (February)
    Year:2013
    Page No: (0-0)
  • Experimental Methods Keywords

    Methodology:Antimicrobial activity
    Research Materials:Silver nanoparticles

Keywords

Silver nanoparticles Photo-irradiation Streptomyces coelicolor Antimicrobial activity

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