Journal: Journal of Pharmacy Research

Article Id: JPRS-PB-00001291
Title: Molecular identification of amylase producing Bacillus subtilis and detection of optimal conditions
Category: Pharmaceutical Biotechnology
Section: Research Article
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    Background: Amylase is of great significance in industrial applications like pharmaceutical, food, textile and paper industries. These enzymes represent approximately 25e33% of the world enzyme market. Methods: Potential amylase producing bacterial strains were isolated using SAM from sago industries waste site samples and optimized for different parameters. Results and diccussion: The maximum amylase production (538 U/ml) was achieved when the temperature was around 32 C at pH 7 by the strain SSII2. The production of amylase was found to enhance when hydrolyzed sago starch was added as a sole carbon source, yeast extract as a nitrogen source and cysteine as an amino acid. After optimizing, the isolates scale up studies were carried out in 5 L fermentor with sago starch waste as a sole carbon source. The production of the enzyme reached 2.72 mg/L after 12 h. After partial purification the concentration of alpha amylase was found to be 54.54 mg/L. The maximum amylase enzyme activity was obtained at the beginning of the stationary growth phase. Conclusion: The isolate was identified as Bacillus subtilis after 16S rDNA sequencing and it proved to be a potent amylase producing strain, which can be further used for potential applications in various biotechnological and industrial processes.

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    Author(s) Name:

    D. John Ravindar, Namasivayam Elangovan*

    Affiliation(s) Name:

    Department of Biotechnology, Periyar University, Periyar Palkalai Nagar, Salem 636011, Tamil Nadu, India

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    Author:

    D. John Ravindar, Namasivayam Elangovan*

    Title:Molecular identification of amylase producing Bacillus subtilis and detection of optimal conditions
    Journal:Journal of Pharmacy Research
    Vol(issue):6 (April)
    Year:2013
    Page No: (426-430)
  • Experimental Methods Keywords

    Methodology:Screening of amylase producing bacterial, Effect of incubation time, temperature and pH,ribosomal sequence detection
    Research Materials:Bacillus subtilis

Keywords

Bacillus sp. Industrial enzymes Starch agar medium (SAM) alpha-Amylase

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