Journal: Journal of Pharmacy Research

Article Id: JPRS-PCS-00001298
Title: Development and validation of ratio-derivative spectrophotometric method for simultaneous estimation of Gabapentin, Methylcobalamin and alpha lipoic acid in tablet formulation
Category: Pharmaceutics
Section: Research Article
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    Aim: The purpose of this study is to develop simple, economic, specific, rapid, reliable, and reproducible method for simultaneous estimation of the Gabapentin, Methylcobalamin and Alpha lipoic acid. Method: In this method the overlapping spectra of Gabapentin, Methylcobalamin and Alpha lipoic acid were well resolved by making use of the first-derivative of the ratios of their direct absorption spectra. The derivative ratio absorbance of Gabapentin, Methylcobalamin and Alpha lipoic acid were measured at 731.10 nm, 768.53 nm and 242.21 nm for their quantification. The method was validated for accuracy, precision, linearity, robustness and sensitivity. Result & discussion: Gabapentin, Methylcobalamin and Alpha lipoic acid were shown linearity in the concentration range of 100-500 mg/ml, 0.5e2.5 mg/ml and 100e500 mg/ml respectively. The LOD & LOQ were found to be 3.09 mg/ml and 9.37 mg/ml; 0.03 mg/ml and 0.10 mg/ml; and 4.79 mg/ml and 14.52 mg/ml respectively. The % labelled claim for Gabapentin, Methylcobalamin and Alpha lipoic acid were found to be 98.71, 98.94 and 98.44 respectively. Conclusion: Thus, the described method is suitable for routine analysis and quality control of pharmaceutical preparations containing these drugs in combination.

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    Author(s) Name:

    Pratik Pravinbhai Goti a,*, Parula B. Patel b

    Affiliation(s) Name:

    a Research Scholar, Department of Quality Assurance, S J Thakkar Pharmacy College, Nr. NRI Bungalow, Avadh Road, Kalawad Road, Rajkot 360005, Gujarat, India 
    b Associate Professor, Department of Quality Assurance, S J Thakkar Pharmacy College, Rajkot 360005, Gujarat, India

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    Author:

    Pratik Pravinbhai Goti a,*, Parula B. Patel b

    Title:Development and validation of ratio-derivative spectrophotometric method for simultaneous estimation of Gabapentin, Methylcobalamin and alpha lipoic acid in tablet formulation
    Journal:Journal of Pharmacy Research
    Vol(issue):6 (June)
    Year:2013
    Page No: (609-614)
  • Experimental Methods Keywords

    Methodology:Development , Validation, Spectra Spectrophotometry
    Research Materials:Alpha lipoic acid, Gabapentin, Methylcobalamin

Keywords

Alpha lipoic acid Gabapentin Methylcobalamin Ratio derivative spectra Spectrophotometry

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