Journal: Journal of Pharmacy Research

Article Id: JPRS-PB-00001299
Title: Efficacy of Helicteres isora L. against free radicals, lipid peroxidation, protein oxidation and DNA damage
Category: Pharmaceutical Biotechnology
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    Background: Free radicals are implicated with cellular disorders through their detrimental actions on proteins, lipids and DNA and are causative factors for large number of degenerative diseases and aging process. Antioxidants of plant origin hold great significance and have therefore gained utmost importance in recent past. Present investigation was a step in this direction, with an objective to comprehensively evaluate the protective effects of Helicteres isora fruits against free radicals, protein oxidation and DNA damage. Methods: Aqueous (AqE), aqueous-methanol (AqME), methanol (ME) and acetone (AE) extracts of mature pods (fruits) were obtained and concentrated in vacuo. Total phenols, flavonoids, ascorbic acid and carotenoids were estimated from extracts using standard methods. Antioxidant activities of extracts with varying concentrations (200-1000 mg/ml) were determined by total antioxidant activity (TAA), ferric reducing antioxidant power, DPPH, and OH radical scavenging assays besides lipid peroxidation inhibition. Extracts were assessed for protection against AAPH (2,20-Azobis(2-methylpropionamidine) dihydrochloride) induced-protein oxidation using SDS-PAGE and Fenton’s reagent induced- DNA damage using DNA nicking assay. Results: The results postulated that the plant is a rich source of total phenols, flavonoids and ascorbic acid. Extracts showed concentration-dependent free radical scavenging activities and lipid peroxidation inhibition. Amongst all four extracts, AqME showed highest antioxidant potential in terms of reducing power (360  5.9 gallic acid equivalent: GAE), TAA (150  5.6 GAE), scavenging of free radicals including DPPH (75.6%) and OH (100%) besides maximal (97.4%) lipid peroxidation inhibition at 1000 mg/ml concentration. All the extracts barring ME effectively protected the DNA from hydroxyl radical-induced damage. Similarly, fruit extracts effectively protected the AAPH-induced-protein oxidation. Conclusions: H. isora fruits exhibited broad-spectrum antioxidant potencies against free radicals and significantly ameliorated various impairments associated with free radical formation including lipid peroxidation, protein oxidation and DNA damage.

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    Author(s) Name:

    Vinay Kumar a,*, Mansi Sharma a, Melissa Lemos a, Varsha Shriram b

    Affiliation(s) Name:

    a Department of Biotechnology, Modern College, Ganeshkhind, Pune 411053, Maharashtra, India 
    b Department of Botany, Prof. Ramkrishna More College, Akurdi, Pune 411044, Maharashtra, India

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    Author:

    Vinay Kumar a,*, Mansi Sharma a, Melissa Lemos a, Varsha Shriram b

    Title:Efficacy of Helicteres isora L. against free radicals, lipid peroxidation, protein oxidation and DNA damage
    Journal:Journal of Pharmacy Research
    Vol(issue):6 (June)
    Year:2013
    Page No: (620-625)
  • Experimental Methods Keywords

    Methodology:DPPH, Fenton’s reaction, Free radicals, Lipid peroxidation
    Research Materials:Helicteres isora L.

Keywords

DPPH Fenton’s reaction Free radicals Lipid peroxidation

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