Journal: Journal of Pharmacy Research

Article Id: JPRS-BMB-00001305
Title: A detail study of phytochemical screening, antioxidant potential and acute toxicity of Agaricus bisporus extract and its chitosan loaded nanoparticles
Category: Biochemistry and Molecular Biology
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    Aim: Scrutinize the phytochemical, antioxidant and acute toxicity of Agaricus bisporus extract (ABE) and A. bisporus loaded chitosan nanoparticles (ABCNPs). Methods: Phytochemical such as total phenol and flavonoid, and terpenoid, alkaloid, steroid, carbohydrate, tannins and proteins where determined. The antioxidant activity such as DPPH, ABTS and reducing power of ABE and ABCNPs where estimated by spectrophotometry assay. The estimation of total phenolic content was determined by Foline Ciocalteu method. The acute toxicity studies of ABE and ABCNPs were investigated in male Sprague Dawley rats. Results: In the DPPH, ABTSþ and reducing power scavenging assays, of ABE displayed significant antioxidant activities with the inhibition values of 27.78, 27.62 and 81.97% and ABCNPs inhibition values 27.78, 27.62 and 78.13% respectively. The reducing power of the extract increased dose-dependently, and the ABE and ABCNPs reduced the most Fe3þ ions. The amount of total phenolics was reported 1 g of sample contains 8.191 mg of gallic acid and total flavonoid analysis by the assay of aluminum chloride spectrophotometric reported 1 g of sample contains 10.3  1 mg of quercetin in ABE and ABCNPs. The acute toxicity was found bellow 2747.25 mg/kg b.w. in ABE and 3178.86 mg/kg b.w. of ABCNPs. Conclusion: The white button mushroom A. bisporus could be considered as a dietary natural food products with antioxidant activity. Thus our results provide evidence that AB and ABCNPs proves to have a potent antioxidant and intermittent therapy against cancer.

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    Author(s) Name:

    Ganesan Dhamodharan, Sankaran Mirunalini*

    Affiliation(s) Name:

    Department of Biochemistry & Biotechnology, Annamalai University, Annamalai Nagar, 608 002 Tamil Nadu, India

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    Author:

    Ganesan Dhamodharan, Sankaran Mirunalini*

    Title:A detail study of phytochemical screening, antioxidant potential and acute toxicity of Agaricus bisporus extract and its chitosan loaded nanoparticles
    Journal:Journal of Pharmacy Research
    Vol(issue):6(8)
    Year:2013
    Page No: (818-822)
  • Experimental Methods Keywords

    Methodology:Chitosan nanoparticles, Free radicals,Antioxidants,DPPH, ABTS and reducing power
    Research Materials:Agaricus bisporus

Keywords

Antioxidants Agaricus bisporus Chitosan nanoparticles Free radicals

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