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Abstract

Background: Juice of Tinospora cordifolia is well reported for its immuno-modulatory and adaptogenic properties. Importance of the plant is well understood as its stem juice was widely used during the Swine flu break in India as an immuno-modulator. On the contrary, there are no data available on the shelf-life of juice. Therefore, effects of cold storage on the phytochemical profiles of T. cordifolia stem juice were analyzed. Methods: Juices of mature stems of the T. cordifolia were prepared and stored at 0
C. The juice samples were analysed on 0, 15 and 30 days intervals using UPLC-QTOFMS. The juice samples were resolved on C-18 (4.6  250 mm, 1.8 mm) column at 40
C. The optimized method provided a good linear relation (r  0.9213 for all the internal standards), satisfactory precision (RSD values less than 2.1%) and good recovery (97.3-101.2%). Results and discussion: Initial analysis of MSn data showed presence of 14,101 molecular features in the samples. Variation in the molecular features analysed using principal component analysis and discrimination models showed 99% variation across the samples analysed on 0, 15 and 30 day. Jatrorrhizine, mangoflorine, menisperine, columbamine, berberine and tinosporoside along with other most abundant metabolites were degraded 70-80% over a period of 15 days and 99% in 30 days. High degradation rate of metabolites constituents was observed may be due to the enzymes present in the juice or oxidative reactions. Therefore, it is recommended to use the fresh juice of T. cordifolia and stability of its herbal formulation should be analysed carefully.

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	JPR Solutions

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