Journal: Journal of Pharmacy Research

Article Id: JPRS-PCS-00001338
Title: RP-HPLC Method Development and Validation for Estimation of Thiocolchicoside and Diclofenac Potassium in Bulk and Capsule Dosage Forms
Category: Pharmaceutics
Section: Research Article
  • Abstract
  • Audio Abstract
  • Authors
  • Pdf File
  • Citation
  • My Reference
  • Methodology
  • Abstract

    A new simple, specific, precise and accurate reversed-phase liquid chromatography method has been developed for simultaneous estimation of Thiocolchicoside (THC) and Diclofenac potassium (DICP) in capsule formulation. The separation were achieved on a 5-micron C18 column (250 X 4.6 mm) using mobile phase consisting of a mixture of Acetonitrile: Methanol: water (35:15;50), pH adjusted to 3.5 with orthophosphoric acid. The flow rate was maintained at 1.0 ml/min, with an average operating pressure of 2630 psi. The detection of the constituents was done using UV detector at 286.0 nm for THC and DICP. The retention time of THC and DICP were approximately 3.3 and 4.0 min respectively. Recovery study values of THC and DICP is 100.09+0.30 and 99.91+0.29 respectively, relative standard deviation of less than 2% for the assay and linearity coefficient of 0.999 shows that the method is precise, accurate and linear in the concentration given and demonstrate the method developed is rugged and robust. Linear response obtained for THC was in the concentration range 20-100 μg/ml and DICP in the range 20-100 μg/ml.

  • Abstract Audio

    Click Here

  • About the authors and Affiliations

    Author(s) Name:

    A.R.Umarkar*, N.S.Rewatkar, M.S.Charde, R.M. Charde, A.V.Kasture

    Affiliation(s) Name:

    J.L.Chaturvedi College of Pharmacy, Electronic zone building, MIDC Hingna Road Nagpur M.S. India. 404016

    *Corresponding author.
    A.R.Umarkar
    J.L.Chaturvedi College of Pharmacy,
    Electronic zone building,
    MIDC Hingna Road ,Nagpur,
    M.S. India. 404016


    Received on: 05-12-2010; Revised on: 14-01-2011; Accepted on:09-03-2011

  • View Article File in pdf format.

    Article File
  • View Article Citation Here.

    9 View More
  • How to Cite my Article.

    Author:

    A.R.Umarkar*, N.S.Rewatkar, M.S.Charde, R.M. Charde, A.V.Kasture

    Title:RP-HPLC Method Development and Validation for Estimation of Thiocolchicoside and Diclofenac Potassium in Bulk and Capsule Dosage Forms
    Journal:Journal of Pharmacy Research
    Vol(issue):4 (May)
    Year:2011
    Page No: (1307-1308)
  • Experimental Methods Keywords

    Methodology:RP-HPLC
    Research Materials:Thiocolchicoside, Diclofenac potassium

Keywords

Thiocolchicoside Diclofenac potassium and RP-HPLC

Our Services

Most Downloaded List