Journal: Journal of Pharmacy Research

Article Id: JPRS-MB-00001350
Title: Phytochemical screening and in vitro antimicrobial activity of Typha angustifolia Linn leaves extract against pathogenic gram negative micro organisms
Category: Microbiology
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    Background: Typha is a monocot genus of the monotypic family Typhaceae with about 12 species distributed in the tropical and temperate regions of the world in marshes and wetlands of varied depth. It a common plant of wetlands, is an unexploited taxon which can be used as a good source of food, medicines, and, fibres. Methods: In the present study phytochemical evaluation and antimicrobial potency of  Typha angustifolia has been investigated against 4 g negative standard reference bacterial strains i.e., Enterobacter aerogenes (MTCC111), Salmonella typhimurium (MTCC98), Klebsiella pneumonia (MTCC 109), Pseudomonas aeruginosa (MTCC 424) and Escherichia coli (clinical isolates). The antibacterial activity was determined by agar well diffusion method and broth dilution methods were used to determine the minimum inhibitory concentration (MIC) of the above strains. Results: It is estimated that total ash value in leaves is 10.83%, acid insoluble ash and water soluble ash shows the value 4.66% and 3.16% respectively. Preliminary phytochemical screening of the aerial part of the T. angustifolia reveals the presence of different secondary metabolites in these extracts. The diameter of the zones of inhibition of the extracts ranged from 13 to 17 mm for E. aerogenes, 0e19 for S. typhimurium, 12e16 for K. pneumoniae, 9-19 for P. aeruginosa and 9e20 mm for E coli. The minimum inhibitory concentration (MIC) of the different extracts ranged from 0.78 mg/ml to 12.5 mg/ml. Conclusion: The results obtained with methanol extract was particularly significant as it strongly inhibited the growth of S. typhimurium, P. aeruginosa and E. coli. The overall results of this study provided informative data for the use of T. angustifolia for the treatment of infections associated with the studied micro organisms.

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    Author(s) Name:

    Ramesh L. Londonkar*, Umesh Madire Kattegouga, Kirankumar Shivsharanappa,
    Jayashree V. Hanchinalmath

    Affiliation(s) Name:

    Department of P.G. Studies and Research in BioTechnology, Gulbarga University, Jnana Ganga Campus, Sedam Road, Gulbarga 585106, Karnataka, India

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    Author:

    Ramesh L. Londonkar*, Umesh Madire Kattegouga, Kirankumar Shivsharanappa,
    Jayashree V. Hanchinalmath

    Title:Phytochemical screening and in vitro antimicrobial activity of Typha angustifolia Linn leaves extract against pathogenic gram negative micro organisms
    Journal:Journal of Pharmacy Research
    Vol(issue):6 (February)
    Year:2013
    Page No: (280-283)
  • Experimental Methods Keywords

    Methodology:Antimicrobial, Phytochemical study
    Research Materials:Typha angustifolia

Keywords

Antimicrobial Gram negative bacteria Phytochemical Typha angustifolia

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