Journal: Journal of Pharmacy Research

Article Id: JPRS-PCS-00001371
Title: RP-HPLC Method Development and Validation for Simultaneous Estimation of Amlodipine besylate,Valsartan and Hydrochlorothiazide in Tablet Dosage Form
Category: Pharmaceutics
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  • Abstract

    A simple, specific, accurate and precise Reverse Phase High Performance Liquid Chromatographic method was developed for simultaneous estimation of Amlodipine Besylate (AB), Valsartan (VAT) and Hydrochlorothiazide (HTZ) in tablet dosage form on RP C-18 Column (Hypersil 250*4.6 mm) using Acetonitrile: Mixed Phosphate buffer (6.8 pH) (55:45) ( buffer was prepared by mixing the equal proportions of 0.01M Potassium dihydrogenphosphate and 0.001M Dipotassium hydrogenphosphate. pH was adjusted with Orthophosphoric acid) as mobile phase. The flow rate was 1.0 ml/min and effluent was monitored at 237nm. The retention time for VAT, HTZ and AB was found to be as 2.28, 2.99 and 4.57 respectively. Proposed method was validated for Precision, Accuracy, Linearity range, Robustness and Ruggedness.

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    Author(s) Name:

    Mohammad Younus1*, T. Karnaker Reddy1, Y. Ravindra Reddy1, Md. Fasiuddin Arif2

    Affiliation(s) Name:

    1. Bharat Institute of Technology (Pharmacy), Ibrahim Patnam, Rangareddy (Dist), A.P, India.
    2. Alphamed Formulations Pvt.Ltd, Shamirpet, Rangareddy (Dist), A.P, India.

    *Corresponding author.
    Mohammad Younus
    Bharat Institute of Technology (Pharmacy), Ibrahim Patnam, Rangareddy
    (Dist), A.P, India.


    Received on: 15-06-2010; Revised on: 18-08-2010; Accepted on:13-09-2010

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    Author:

    Mohammad Younus1*, T. Karnaker Reddy1, Y. Ravindra Reddy1, Md. Fasiuddin Arif2

    Title:RP-HPLC Method Development and Validation for Simultaneous Estimation of Amlodipine besylate,Valsartan and Hydrochlorothiazide in Tablet Dosage Form
    Journal:Journal of Pharmacy Research
    Vol(issue):3(11)
    Year:2010
    Page No: (2647-2650)
  • Experimental Methods Keywords

    Methodology:Reverse Phase High Performance Liquid Chromatography
    Research Materials:Amlodipine Besylate, Valsartan, Hydrochlorothiazide

Keywords

Amlodipine Besylate Valsartan Hydrochlorothiazide Reverse Phase High Performance Liquid Chromatography

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