Journal: Journal of Pharmacy Research

Article Id: JPRS-ND-00001455
Title: In vitro radical scavenging activity and phytochemical screening for evaluation of the antioxidant potential of Operculina turpethum root extract
Category: Natural Drugs
Section: Research Article
  • Abstract
  • Audio Abstract
  • Authors
  • Pdf File
  • Citation
  • My Reference
  • Methodology
  • Abstract

    Cellular damage or oxidative injury arising from free radicals or reactive oxygen species (ROS) now appears the fundamental mechanism underlying a number of human neurodegenerative disorders, diabetes, inflammation, viral infections, autoimmune pathologies and digestive system disorders. Naturally occurring antioxidant supplements from plants are vital to counter the oxidative damage in cells. We assessed the antioxidant potential and phytochemical constituents of ethanolic extract of Operculina turpethum using tests involving inhibition of H2O2, superoxide anions, reducing power and FRAP . The phenolic, flavonoid and proanthocyanidin contents of the extract were also determined using standard phytochemical reaction methods. The results of the assay were then compared with natural antioxidants Quercitin, rutin and BHA. On the basis of present investigation the extract showed high significance (p<0.001) as compared to standards. Operculina turpethum extract showed the presence of alkaloids, carbohydrates, saponins, flavonoids and cardiac glycosides. A positive correlation between the antioxidant activities and physiochemical assays was observed and the highest scavenging activity of extract was noticed at concentration of 1mg/ml.. These finding suggest that this plant is a potential source of natural antioxidant that could serve as free radical inhibitors or scavenger, could have great importance as therapeutic agents, anti-inflammatory, anti-analgesic, anti-anaemic, in preventing or slowing the progress of ageing and age associated oxidative stress related degenerative diseases such as cancer and various other human ailments.

  • Abstract Audio

    Click Here

  • About the authors and Affiliations

    Author(s) Name:

     Veena Sharma* and  Manu Singh

    Affiliation(s) Name:

    Department of Bioscience and Biotechnology, Banasthali University, Banasthali 304022, Rajasthan, India

    *Corresponding author.
    Dr. Veena Sharma
    Department of Bioscience and Biotechnology,
    Banasthali University, Banasthali 304022,
    Rajasthan, India

    Received on:10-11-2011; Revised  on: 15-12-2011; Accepted on:12-01-2012

  • View Article File in pdf format.

    Article File
  • View Article Citation Here.

    9 View More
  • How to Cite my Article.

    Author:

     Veena Sharma* and  Manu Singh

    Title:In vitro radical scavenging activity and phytochemical screening for evaluation of the antioxidant potential of Operculina turpethum root extract
    Journal:Journal of Pharmacy Research
    Vol(issue):5 (February)
    Year:2012
    Page No: (783-787)
  • Experimental Methods Keywords

    Methodology:Anticancer, Antioxidant, BHA, FRAP
    Research Materials:Operculina turpethum

Keywords

Anticancer Antioxidant BHA FRAP Operculina turpethum

Our Services

Most Downloaded List