Journal: Journal of Pharmacy Research
Title: In vitro radical scavenging activity and phytochemical screening for evaluation of the antioxidant potential of Operculina turpethum root extract
Category: Natural Drugs
Section: Research Article
- Abstract
- Audio Abstract
- Authors
- Pdf File
- Citation
- My Reference
- Methodology
-
Abstract
Cellular damage or oxidative injury arising from free radicals or reactive oxygen species (ROS) now appears the fundamental mechanism underlying a number of human neurodegenerative disorders, diabetes, inflammation, viral infections, autoimmune pathologies and digestive system disorders. Naturally occurring antioxidant supplements from plants are vital to counter the oxidative damage in cells. We assessed the antioxidant potential and phytochemical constituents of ethanolic extract of Operculina turpethum using tests involving inhibition of H2O2, superoxide anions, reducing power and FRAP . The phenolic, flavonoid and proanthocyanidin contents of the extract were also determined using standard phytochemical reaction methods. The results of the assay were then compared with natural antioxidants Quercitin, rutin and BHA. On the basis of present investigation the extract showed high significance (p<0.001) as compared to standards. Operculina turpethum extract showed the presence of alkaloids, carbohydrates, saponins, flavonoids and cardiac glycosides. A positive correlation between the antioxidant activities and physiochemical assays was observed and the highest scavenging activity of extract was noticed at concentration of 1mg/ml.. These finding suggest that this plant is a potential source of natural antioxidant that could serve as free radical inhibitors or scavenger, could have great importance as therapeutic agents, anti-inflammatory, anti-analgesic, anti-anaemic, in preventing or slowing the progress of ageing and age associated oxidative stress related degenerative diseases such as cancer and various other human ailments.
-
Abstract Audio
About the authors and Affiliations
Author(s) Name: Veena Sharma* and Manu Singh
Affiliation(s) Name: Department of Bioscience and Biotechnology, Banasthali University, Banasthali 304022, Rajasthan, India
*Corresponding author.
Dr. Veena Sharma
Department of Bioscience and Biotechnology,
Banasthali University, Banasthali 304022,
Rajasthan, IndiaReceived on:10-11-2011; Revised on: 15-12-2011; Accepted on:12-01-2012
-
View Article File in pdf format.
Article File
-
View Article Citation Here.
9 View More -
How to Cite my Article.
Author: Veena Sharma* and Manu Singh
Title: In vitro radical scavenging activity and phytochemical screening for evaluation of the antioxidant potential of Operculina turpethum root extract Journal: Journal of Pharmacy Research Vol(issue): 5 (February) Year: 2012 Page No: (783-787) -
Experimental Methods Keywords
Methodology: Anticancer, Antioxidant, BHA, FRAP Research Materials: Operculina turpethum
Keywords
Our Services
- In vitro antioxidant and ptp inhibitory activity of methanolic extract of Anogeissus acuminata leaf and bark.
- Solubility and Dissolution Enhancement: An overview
- Ethosomes: A novel tool for drug delivery through the skin
- A review on Calotropis procera Linn and its Ethnobotany, Phytochemical, Pharmacological profile
- Antimicrobial activity and phytochemical screening of five wild plants against Escherichia coli, Bacillus subtilis and Staphylococcus aureus
- A review on quality by design approach for analytical method development
- Development and Validation of Stability Indicating UV Spectrophotometric Method for the Estimation of Sitagliptin Phosphate in Bulk and Tablet Dosage Form
- Evaluation of Phenolic Content and Antioxidant Capacity of leaf extract from Phoenix Dactylifera L obtained by different pH of aqueous extraction
- Benzoic acid, one of the major components of aqueous bark extract of Terminalia arjuna protects against Copper-Ascorbate induced oxidative stress in human placental mitochondria through antioxidant m
- Zinc-silibinin complex ameliorates oxidative stress in high fat fed low dose STZ induced type 2 diabetes in rats
- Biological synthesis of silver nanoparticles using aqueous leaf extract of Capparis decidua (FORSK.) EDGEW: A better alternative
- Formulation and evaluation of topical gel of diclofenac sodium using different polymers
- Prescribing Patterns of antihypertensive drugs in a South Indian tertiary care hospital
- Study of health related quality of life in rheumatoid arthritis patients
- In vitro antioxidant, anti-inflammatory and anticholinesterase activities of Rumex vesicarius L
- Antimicrobial activity of seaweeds Gracillaria, Padina and Sargassum sps. on clinical and phytopathogensSubba Rangaiah G, Lakshmi P* and Manjula E
- Antidiabetic activity of insulin plant (Costus igneus) leaf extract in diabetic rats
- Gastroprotective effect of Fenugreek 4-hydroxyisoleucine and trigonelline enriched fraction (TF4H (28%)) Sugaheal® against indomethacin induced ulcer in male wistar rats.
- In vitro anti-Candida activity of Calotropis gigantea.
- Pharmacognostic, Phytochemical & Quantitative Investigation of Saraca asoca leaves
- Anti ulcer activity of Naravelia zeylanica leaves extract
- Melatonin affords protection against myocardial ischemia-induced cerebral mitochondrial dysfunction: an in vivo study.
- Effects of Osmolytes on The Structural Stability of Bovine Trypsin: A Brief Review
- Synthesis and Biological Activity of Novel Acyl Hydrazone Derivatives of 3-(4,5-diphenyl-1,3-oxazol-2-yl) propanoic acid as Anticancer, Analgesic and Anti-inflammatory Agents
- Design and Synthesis of Novel Quinazolinone Derivatives as Broad Spectrum Anticonvulsant and Antimicrobial Agent
- In vitro Antioxidant Activity from Leaves of Oroxylum indicum (L.) Vent. -A North Indian Highly Threatened and Vulnerable Medicinal Plant
- Assessment of Pharmacognostical Characters of Leaves of Tephrosia Purpurea Linn
- Genome of Acinetobacter baumannii Carries Multi Drug Resistance Genes
- Triazole analogues as novel antioxidants: In vitro bioassay and molecular modeling studies
- Anticonvulsant And Antioxidant Actions Of Some Dibenzo-a-Pyrone Derivatives In Pentylene –Induced Kindling Model In Mice