Journal: Journal of Pharmacy Research

Article Id: JPRS-MB-00001587
Title: Antimicrobial potential of Nyctanthes arbor-tristis and isolation of Colletotrichum gleosporioides –an endophyte.
Category: Microbiology
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    A comparative study has been carried out to assess antibacterial potential of different plant parts of Nyctanthes arbor-tristis. Leaf was experienced as the most efficient plant part having antibacterial activity against pathogen tested. Antibacterial activity of aqueous and methanolic extract of root, shoot and leaf were tested by well diffusion method. Leaf was taken for further activity by preparing four fractions (ethyl acetate, n- butanol, chloroform and aqueous) of its’ methanolic extract. Antibacterial activity of these fractions were analysed for more accuracy. Sections of leaf of N. arbor-tristis were inoculated on PDA media to isolate and identify endophytic fungi. Aqueous extract of leaf possess greater activity against pathogenic bacteria than methanolic extract. Ethyl acetate, n- butanol  and aqueous fraction of methanolic leaf extract is the effective fraction having antibacterial activity against Klebsiella pneumoniae, Staphylococcus epidermidis, Listeria monocytogens, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. On the basis of morphological features, biomass production and enzymatic studies the isolated endophytic fungus was identified as Colletotrichum gleosporioides.

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    Author(s) Name:

    Chouhan Ankita, Gokhale Mamta* &  Bansal Shikha

    Affiliation(s) Name:

    Department of Botany & Microbiology,St. Aloysius (autonomous) College, Jabalpur-M.P.-India

    *Corresponding author.
    Gokhale Mamta
    Department of Botany & Microbiology
    St. Aloysius (autonomous) College,
    Jabalpur-M.P.-India

    Received on:26-05-2014; Revised  on: 12-06-2014; Accepted on:31-07-2014

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    Author:

    Chouhan Ankita, Gokhale Mamta* &  Bansal Shikha

    Title:Antimicrobial potential of Nyctanthes arbor-tristis and isolation of Colletotrichum gleosporioides –an endophyte.
    Journal:Journal of Pharmacy Research
    Vol(issue):8(8)
    Year:2014
    Page No: (1082-1090)
  • Experimental Methods Keywords

    Methodology:phytochemical test, antimicrobial activity, pathogenic bacteria, endophytic fungus, Colletotrichum gleosporioides
    Research Materials:Medicinal plant, Nyctanthes arbor-tristis

Keywords

Medicinal plant Nyctanthes arbor-tristis phytochemical test antimicrobial activity pathogenic bacteria endophytic fungus Colletotrichum gleosporioides

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