Journal: Journal of Pharmacy Research

Article Id: JPRS-PHDRS-00001588
Title: Antibacterial potential of Elletaria cardamomum, Syzygium aromaticum and Piper nigrum, their synergistic effects and phytochemical determination
Category: Pharmacognosy and Herbal Drugs related study
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    Background: Spices are considered as rich source of bio-active antimicrobial compounds and are indispensable components of Indian cuisines since ancient times. To provide a scientific basis to traditional uses of Elletaria cardamomum, Syzygium aromaticum and Piper nigrum, their aqueous and organic seed extracts, isolated phytoconstituents and combinations were evaluated for antibacterial potential against gram positive (Staphylococcus aureus, Bacillus subtilis) and gram negative (Escherichia coli, Salmonella typhi) bacteria.  Methods: Antibacterial activity of aqueous and organic seed extracts was assessed using agar diffusion assay, minimum inhibitory concentration and the effect was compared with some standard antibiotics. The presence of major phytoconstituents was detected qualitatively and quantitatively. The isolated phytoconstituents and combination of spices were subjected to disc diffusion assay to ascertain their antibacterial effect.
    Results: Organic extracts of all three spices showed good antibacterial activity against all the test strains, which was found to be comparable with standard antibiotics. Minimum inhibitory concentration for aqueous and organic seed extracts ranged from 25 to >50 mg/ml and 2 to 50 mg/ml respectively. Inhibitory activity of all the extracts was found to be increased when used in combination. This synergistic effect supports the use of these spices in combination. Quantitative phytochemical analysis showed the presence of 2.30 – 7.8% alkaloids, 5.7 – 26.2% flavonoids, 11.0 – 33.0% tannins, 0.60 – 3.8% saponins. Conclusion: Antibacterial efficacy shown by these spices and their combinations provides a scientific basis and thus, validates their traditional uses as homemade remedies. Isolation and characterization of different phytochemicals may further yield significant antibacterial agents.

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    Author(s) Name:

    Nomita Gupta, Palak Parashar, Monika Mittal, Varsha Mehra, Manisha Khatri*

    Affiliation(s) Name:

    Department of Biomedical Science, Shaheed Rajguru College of Applied Sciences for Women, University of Delhi, India

    *Corresponding author.
    Dr. Manisha Khatri,
    Assistant Professor,
    Department of Biomedical Science,
    Shaheed Rajguru College of Applied Sciences for Women,
    University of Delhi, Delhi, India

    Received on:21-05-2014; Revised  on: 17-06-2014; Accepted on:25-07-2014

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    Author:

    Nomita Gupta, Palak Parashar, Monika Mittal, Varsha Mehra, Manisha Khatri*

    Title:Antibacterial potential of Elletaria cardamomum, Syzygium aromaticum and Piper nigrum, their synergistic effects and phytochemical determination
    Journal:Journal of Pharmacy Research
    Vol(issue):8(8)
    Year:2014
    Page No: (1091-1097)
  • Experimental Methods Keywords

    Methodology:Antibacterial activity, Minimum inhibitory concentration, Synergistic effect, Phytoconstituents
    Research Materials:Elletaria cardamomum, Syzygium aromaticum and Piper nigrum

Keywords

Antibacterial activity Minimum inhibitory concentration Synergistic effect Phytoconstituents

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