Journal: Journal of Pharmacy Research

Article Id: JPRS-BGSA-00001590
Title: BLASTN Based Methodology to Classify Benzene Degrading Uncultured Bacteria into Bacterial Species using 16S rRNA Gene
Category: Bioinformatics (Genome & Sequence Analysis )
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  • Abstract

    Background: Benzene degradation is an important factor in pollution treatment studies as benzene is listed as frequently used chemical in an industry. Methods: During investigation we have referred several uncultured bacteria bringing about the degradation of benzene but cannot be identified for its taxonomical classification. We have developed a protocol using BLASTN and its associated 16S rRNA database, using it a precise bacterial species could be linked to the uncultured bacteria investigated by sequence homology. Results and Discussion:  Here we have reported 102 bacterial sp. with its genus and species being capable of degrading benzene which was previously being categorized as uncultured bacteria as per the information available in public nucleotide database. Conclusion: BLASTN based protocol can successfully classify the uncultured bacterium based on the 16S rRNA gene sequence by using the 16S rRNA gene database instead of using the nr database linked in BLAST program.

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    Author(s) Name:

    Shefalee Satija1 and Dilip Gore 1*

    Affiliation(s) Name:

    1Sai Bioinfosys Institute of Bioinformatics Research, Nagpur, Maharashtra, India.

    *Corresponding author.
    Dilip Gore
    Sai Bioinfosys Institute of Bioinformatics Research,
    Nagpur, Maharashtra, India.

    Received on:21-05-2014; Revised  on: 17-06-2014; Accepted on:25-07-2014

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    Author:

    Shefalee Satija1 and Dilip Gore 1*

    Title:BLASTN Based Methodology to Classify Benzene Degrading Uncultured Bacteria into Bacterial Species using 16S rRNA Gene
    Journal:Journal of Pharmacy Research
    Vol(issue):8(8)
    Year:2014
    Page No: (1105-1112)
  • Experimental Methods Keywords

    Methodology:BLASTN ,16S rRNA database
    Research Materials:Bacterial Identification

Keywords

Bacterial Identification Benzene degradation BLASTN Uncultured bacterium.

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