Journal: Journal of Pharmacy Research

Article Id: JPRS-P'Col-00001593
Title: Silibinin treatment to hepatic stellate cells: an exploration of apoptosis and cellular senescence
Category: Pharmacology
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    Objective: Proliferation and activation of hepatic stellate cells play pivotal role in the progression of hepatic fibrosis consequent to chronic liver injury. Thus, the inhibition and clearance of activated HSCs from the injured liver by apoptosis and cellular senescence is clearly an appropriate approach for therapeutic treatment for hepatic fibrosis. To evaluate the pro-apoptotic and cellular senescence inducing potential of silibinin, a flavonoid compound in LX-2 cells. Methods: LX-2 cells treated with various concentration of silibinin (10, 50 and 100 ìM) treatment for 96 h, cytotoxicity test, cell counting pro-apoptotic and cellular senescence induction properties of silibinin were evaluated. Results: The present study demonstrates that SBN treatment shows a dose-dependent fall in active proliferation of immortalized LX-2 cells in serum supplemented medium with out affecting its cell viability. Further, SBN treatments neither induce apoptosis nor cellular senescence in the human hepatic stellate LX-2 cells. Conclusion: The anti-proliferative effect of SBN reported in this study might be due its cell cycle arresting properties. 

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    Author(s) Name:

    Ezhilarasan Devaraj 1, Sivanesan Karthikeyan 2 , Etienne Sokal 1 , Mustapha Najimi 1*

    Affiliation(s) Name:

    1 Institut de Recherche Expérimentale et Clinique (IREC), Laboratory of Pediatric Hepatology and Cell Therapy, Université Catholique de Louvain, Brussels, Belgium.    
    2 Department of Pharmacology and Environmental Toxicology, Food and Hepatotoxicology Laboratory, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Chennai, India.

    *Corresponding author.
    Dr. Mustapha Najimi,                    
    Laboratory of Pediatric Hepatology and Cell Therapy
    Avenue Hippocrate 10/1301
    1200, Brussels, Belgium

    Received on:06-06-2014; Revised  on: 09-07-2014; Accepted on:08-08-2014

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    Author:

    Ezhilarasan Devaraj 1, Sivanesan Karthikeyan 2 , Etienne Sokal 1 , Mustapha Najimi 1*

    Title:Silibinin treatment to hepatic stellate cells: an exploration of apoptosis and cellular senescence
    Journal:Journal of Pharmacy Research
    Vol(issue):8(8)
    Year:2014
    Page No: (1126-1131)
  • Experimental Methods Keywords

    Methodology:hepatic stellate cells, apoptosis, cellular senescence, proliferation
    Research Materials:silibinin

Keywords

silibinin hepatic stellate cells apoptosis cellular senescence proliferation

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