Journal: Journal of Pharmacy Research

Article Id: JPRS-PB-00001599
Title: Quantitative determination of Eugenol in aqueous extract of Ocimum sanctum by High Performance Thin Layer Chromatography
Category: Pharmaceutical Biotechnology
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    Background: The present study describes development and validation of new, rapid, accurate and precise, high performance thin layer chromatographic (HPTLC) method for quantitative determination of eugenol in herbal extract of Ocimum sanctum with densito-metric detection. Methods: High-performance thin layer chromatography (HPTLC) studies were carried out using CAMAG HPTLC system equipped with Linomat V applicator, TLC scanner 3 and winCATS 3 software were used. HPTLC on aluminium-backed silica gel 60 F254 plates with toluene-ethyl acetate-formic acid, 90: 10: 01 (v/v) as mobile phase was followed by densito-metric measurement at 280 nm. Results and Discussion: This system was found to give compact bands for eugenol (RF 0.59). The accuracy of the method was checked by conducting recovery studies for two different levels of eugenol; the average recovery was found to be 98.39%. The average eugenol content, as estimated by use of the proposed method, was 46.6 ng µL-1. Conclusion: The method was found to be simple, sensitive, selective and accurate. This study reports a routine quantitative method for the analysis of eugenol, in the leaf extract of Ocimum sanctum.

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    Author(s) Name:

    Nargis Khan and Sharique A Ali*

    Affiliation(s) Name:

    Department of Biotechnology, Saifia Science College Bhopal (M.P.) 462001,India.

    *Corresponding author.
    Dr. Sharique A Ali
    Department of Biotechnology,
    Saifia Science College
    Bhopal (M.P.) 462001,India.

    Received on:20-06-2014; Revised  on: 23-07-2014; Accepted on:17-08-2014

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    Author:

    Nargis Khan and Sharique A Ali*

    Title:Quantitative determination of Eugenol in aqueous extract of Ocimum sanctum by High Performance Thin Layer Chromatography
    Journal:Journal of Pharmacy Research
    Vol(issue):8(8)
    Year:2014
    Page No: (1158-1161)
  • Experimental Methods Keywords

    Methodology:HPTLC, Densitometric analysis
    Research Materials:Ocimum sanctum

Keywords

Eugenol Ocimum sanctum HPTLC Densitometric Mobile phase

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