Journal: Journal of Pharmacy Research

Article Id: JPRS-MB-00001606
Title: Phenotypic and Genotypic Characterization of Extended Spectrum beta-Lactamase Producing Escherichia coli Clinical Isolates from Semiurban Area
Category: Microbiology
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    Background: A constant rise in multidrug resistant Escherichia coli, producing extended spectrum ß- lactamases, poses a new problem to health care professionals worldwide, which complicates and limits therapeutic options. Materials and methods: Ninety Escherichia coli isolates were obtained from various clinical specimens from different wards of Rajah Muthiah Medical College and Hospital, Annamalainagar, India. Isolation and identification of isolates were performed using standard procedures. Detection of virulence factors such as hemolysin, slime production and cell surface hydrophobicity were investigated for thirty representative Escherichia coli isolates. Antibiotic resistances profile by Kirby-Bauer disc diffusion method, ESBL detection by standard phenotypic methods and serogrouping were performed for all ninety isolates. Molecular confirmation of ESBL types were performed for thirty representative Escherichia coli using multiplex PCR. Results: Among the thirty representative Escherichia coli isolates, 27(90%) were hydrophobic, 23(76.6%) were slime producers, 4(13.3%) were hemolysin producers, and 22(73.3%) had multiple virulence factors. Of the ninety Escherichia coli isolates 75.5%, 73.3% and 68.8% were resistance to cephataxime, ceftazidime and ceftriaxone respectively. Double disk approximation test and combined disc diffusion method showed 68(75.5%) isolates to be ESBL producers. Predictor disc approximation test revealed 10(11.1%) isolates to be ESBL producers, 58(64.4%) isolates to be both ESBL and AmpC producers. Molecular analysis of thirty representative isolates revealed CTX-M gene present in 30(100%) isolates followed by TEM in 28(93.3%), SHV in 9(30%) and OXA in 8(26.6%) respectively. Conclusion: This present investigation revealed, a high prevalence of plasmid mediated multidrug resistance, which indicates a dire need for effective ESBL surveillance and control and need for urgent search for newer cost effective antimicrobials, especially in rural and semiurban areas of developing nations.

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    Author(s) Name:

    Angamuthu Shalini Maya*1,Dr. Kesani Prabhakar 2,Dr. Luke Elizabeth Hanna 3 ,Dr.Yelavarthi Lakshmi Sarayu 4      

    Affiliation(s) Name:

          
    *1, 2, 4 Division of Medical Microbiology, Rajah Muthiah Medical College &Hospital, Annamalainagar – 608002, Tamil Nadu, India.
    3 Department of Clinical Research, Tuberculosis Research Center, Chetpet, Chennai – 600031, India.
    Received on: 15-09-2010; Revised  on: 18-11-2010; Accepted on:13-12-2010

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    Author:

    Angamuthu Shalini Maya*1,Dr. Kesani Prabhakar 2,Dr. Luke Elizabeth Hanna 3 ,Dr.Yelavarthi Lakshmi Sarayu 4      

    Title:Phenotypic and Genotypic Characterization of Extended Spectrum beta-Lactamase Producing Escherichia coli Clinical Isolates from Semiurban Area
    Journal:Journal of Pharmacy Research
    Vol(issue):4 (January)
    Year:2011
    Page No: (6-10)
  • Experimental Methods Keywords

    Methodology: surface hydrophobicity, slime production, serotypes.
    Research Materials:Clinical Isolates from Semiurban Area

Keywords

ESBL Escherichia coli hemolysin surface hydrophobicity slime production serotypes.

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