Journal: Journal of Pharmacy Research

Article Id: JPRS-PA-00001611
Title: Selective and Validated Spectrophotometric Method for Determination of Acyclovir and Valacyclovir using N-Bromosuccinimide
Category: Pharmaceutical Analysis
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  • Abstract

    Simple, sensitive spectrophotometric methods are presented for the assay of acyclovir and valacyclovir in bulk drug and in tablets. The methods employ N-bromosuccinimide (NBS) as the oxidimetric reagent and dye, methyl orange used as a spectrophotometric reagent. The method involves adding a measured excess of NBS to acyclovir and valacyclovir in acid medium followed by determination of residual NBS by reacting with a fixed amount of methyl orange and measuring the absorbance at 508 nm. The methods developed were applied to the assay of acyclovir and valacyclovir in commercial tablet formulations and the results were compared statistically with those of a reference method. Acyclovir and valacyclovir showed maximum absorbance at 508 nm with linearity was observed in the concentration range of 1-5 µg mL-1 and 5-10 µg mL-1 respectively. The relative standard deviations of 0.024 % for acyclovir and 0.018 % valacyclovir were obtained. The accuracy and reliability of the methods were further ascertained by performing recovery tests via standard-addition method. The recoveries of acyclovir and valacyclovir tablets are in the range 99.26 ±0.52, 99.47±0.96 respectively. The proposed method is simple, rapid, precise and convenient for the assay of acyclovir and valacyclovir in commercial tablet preparations.

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    Author(s) Name:

    T. Anil Kumar, B. M. Gurupadayya*, M.B. Rahul Reddy, M.V. Prudhvi Raju

    Affiliation(s) Name:

    Department of Pharmaceutical Analysis, JSS College of Pharmacy, JSS University, Mysore 570 015, India

    *Corresponding author.
    Dr. BM Gurupadayya
     Department of Pharmaceutical Analysis
     JSS College of Pharmacy, JSS University,
    S.S. Nagar, Mysore-570 015
    Karnataka, India

    Received on: 15-09-2010; Revised  on: 18-11-2010; Accepted on:13-12-2010

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    Author:

    T. Anil Kumar, B. M. Gurupadayya*, M.B. Rahul Reddy, M.V. Prudhvi Raju

    Title:Selective and Validated Spectrophotometric Method for Determination of Acyclovir and Valacyclovir using N-Bromosuccinimide
    Journal:Journal of Pharmacy Research
    Vol(issue):4(1)
    Year:2011
    Page No: (24-27)
  • Experimental Methods Keywords

    Methodology:Spectrophotometry, Pharmaceutical formulation.
    Research Materials:Acyclovir, Valacyclovir

Keywords

Acyclovir Valacyclovir Oxidation N-Bromosuccinimide (NBS) Spectrophotometry Pharmaceutical formulation.

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