Journal: Journal of Pharmacy Research

Article Id: JPRS-PCS-00001620
Title: Simultaneous estimation of citalopram hydrobromide and dothiepin hydrochloride in human plasma by HPLC method
Category: Pharmaceutics
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  • Abstract

     This was a study to develop a rapid, precise, accurate and sensitive high performance liquid chromatographic method for the simultaneous estimation of citalopram hydrobromide and dothiepin hydrochloride in spiked human plasma. The chromatographic method was standardized using a Phenomenex C-18 column (250 x 4.6 mm i.d., 5 µm particle size) with UV detection at 234 nm and flow rate of 1 ml/min. Salbutamol was spiked in plasma as internal standard (IS). The mobile phase consisting of acetonitrile:water (35:65 v/v) containing of 0.01% orthophosphoric acid and 0.01% sodium chloride. The regression equation for citalopram hydrobromide and dothiepin hydrochloride were Y = 0.0282X +0.0750 and Y= 0.0312 X + 0.1187 respectively. LOD and LOQ were found to be 7.978 and 26.595 ng/mlrespectively for citalopram and 11.413 and 38.044 ng/ml respectively for dothiepin. The method was validated and produced accurate and precise results for estimation of the two drugs in human plasma.

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    Author(s) Name:

    V. Dilip Kumar1, Mahesh N. M1, B.M. Gurupadayya2, V. Ravi3

    Affiliation(s) Name:

    1Department of Pharmacology, JSS College of Pharmacy, JSS University, Shivarathreeshwara Nagar, Mysore-570 015.
    2Department of Pharmaceutical Chemistry, JSS College of Pharmacy, JSS University Shivarathreeshwara Nagar, Mysore-570 015.
    3Department of Pharmaceutics, JSS College of Pharmacy, JSS University Shivarathreeshwara Nagar, Mysore-570 015.

    *Corresponding author.
    Dr. B.M. Gurupadayya,
    Department of Pharmaceutical Chemistry,
     JSS College of Pharmacy, JSS University,
     Shivarathreeshwara Nagar, Mysore-570 015


    Received on: 15-09-2010; Revised  on: 18-11-2010; Accepted on:13-12-2010

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    Author:

    V. Dilip Kumar1, Mahesh N. M1, B.M. Gurupadayya2, V. Ravi3

    Title:Simultaneous estimation of citalopram hydrobromide and dothiepin hydrochloride in human plasma by HPLC method
    Journal:Journal of Pharmacy Research
    Vol(issue):4 (January)
    Year:2011
    Page No: (50-52)
  • Experimental Methods Keywords

    Methodology: HPLC analysis
    Research Materials:Citalopram, Dothiepin

Keywords

Citalopram Dothiepin HPLC Plasma Estimation

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