Journal: Journal of Pharmacy Research

Article Id: JPRS-P'Col-00001638
Title: Terminalia arjuna aqueous bark extract protects against cadmium acetate-induced injury to rat liver and heart through antioxidant mechanisms: a dose response study
Category: Pharmacology
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    Background: Cadmium-acetate is a potent hepatotoxic and cardiotoxic heavy metal, which induces oxidative stress by disturbing a number of antioxidant enzymes in rat heart and liver. Terminalia arjuna is an effective antioxidant and free radical scavenger against cadmium acetate induced oxidative stress. The present study was designed to investigate the efficacy of Terminalia arjuna aqueous bark extract in protecting against the cadmium induced oxidative injury in rat heart and liver. Materials and method: The dose and time dependent changes were studied in heart and liver of male Wister rats following the subcutaneous administration of increasing concentrations of cadmium acetate (0.22, 0.44, and 0.88 mg/kg body weight), in every alternate day for a period of 5, 10, and 15 days respectively, to determine the maximum effective dose of cadmium acetate without any mortality. Pre-treatment of rats with aqueous bark extract of Terminalia arjuna (TA) was also studied by oral administration with the increasing doses (10, 20, and 40 mg/kg BW) against cadmium-acetate (0.44mg/kg BW, s.c.,) induced oxidative stress in rat heart and liver to determine the minimum effective dose of TA which can be protected these alterations in both the organs in a concentration dependent manner. The alterations in the activity of the different bio-markers of hepatic and cardiac damage, biomarkers of oxidative stress, and activities of the antioxidant enzymes were studied. Histo-pathological and histo-chemical alterations were also studied through H-E staining, PAS staining and Picrosirius red staining respectively. Structural integrity of hepatic and cardiac tissue were also studied through scanning electron microscopy. Results: The studies revealed that pre-treatment of aqueous bark extract of Terminalia arjuna protects the biomarkers of organ damage, oxidative stress, antioxidant enzymes, from getting altered in the rat heart and liver tissue following treatment with cadmium acetate.  Conclusion: The results of the present study suggest that the treatment of aqueous bark extract of Terminalia arjuna might be beneficial to alleviate cadmium induced toxicity in rat heart and liver.

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    Author(s) Name:

    Bharati Bhattacharjee1, Arnab Kumar Ghosh1, Sanatan Mishra 1, 2 ,
    Jayita Das1, Aindrila Chattopadhyay2, and Debasish Bandyopadhyay1#*

    Affiliation(s) Name:

    1Oxidative Stress and Free Radical Biology Laboratory, Department of Physiology,University of Calcutta, 92, A.P.C. Road, Kolkata-700009, India,
    #Principal Investigator, Centre with Potential for Excellence in a Particular Area (CPEPA), University of Calcutta, University College of Science and Technology, 92, APC Road, Kolkata 700 009, India
    2Department of Physiology, Vidyasagar College, 39, SankarGhosh Lane, Kolkata-700006, India

    *Corresponding author.
    Prof. Debasish Bandyopadhyay
    Oxidative stress and Free Radical Biology Laboratory,
    Department of Physiology, University of Calcutta
    University College of Science and Technology, 92, APC Road, Kolkata 700 009, India.

    Received on:23-09-2016; Revised on: 14-10-2016; Accepted on: 28-11-2016

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    Author:

    Bharati Bhattacharjee1, Arnab Kumar Ghosh1, Sanatan Mishra 1, 2 ,
    Jayita Das1, Aindrila Chattopadhyay2, and Debasish Bandyopadhyay1#*

    Title:Terminalia arjuna aqueous bark extract protects against cadmium acetate-induced injury to rat liver and heart through antioxidant mechanisms: a dose response study
    Journal:Journal of Pharmacy Research
    Vol(issue):10(11)
    Year:2016
    Page No: (771-792)
  • Experimental Methods Keywords

    Methodology:Oxidative stress, Cardiac and hepato protection, Antioxidant efficacy
    Research Materials:Terminalia arjuna (TA)

Keywords

Cadmium acetate (CH3COO) 2 Cd. 2H2O Oxidative stress Cardiac and hepato protection Antioxidant efficacy Terminalia arjuna (TA)

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