Journal: Journal of Pharmacy Research

Article Id: JPRS-P'Col-00001686
Title: Effect of Morinda umbellata Linn. (Rubiaceae) Leaf Extracts on Antigen Induced Mast Cell Degranulation and Active Paw Anaphylaxis in Wistar rats
Category: Pharmacology
Section: Research Article
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    Aim: Morinda umbellata Linn. is a climbing shrub distributed in Khasi Hills, Deccan Peninsula, Eastern Ghats and Western Ghats regions of India up to an altitude of 1500 m. It is an important traditional medicinal plant having curative properties against stomach disorders. The aim of the present study is to scientifically evaluate the mast cell stabilizing and anti-anaphylactic effects of M. umbellata leaves.  Methods: The different solvent extracts of M. umbellata leaves were used to evaluate antigen induced mast cell degranulation and active paw anaphylaxis in Wistar albino rats. Results and Discussion: The leaf ethanolic extract (200 mg/kg and 400 mg/kg), n-hexane extract (200 mg/kg and 400 mg/kg) and chloroform extract at 400 mg/kg doses exhibited significant protection against egg albumin induced peritoneal and mesenteric mast cell degranulation. In mesenteric mast cell study in vitro, the ethanolic and n-hexane leaf extracts (2 mg/mL) showed significant activity against egg albumin induced mast cell degranulation. In active paw anaphylaxis, the animals treated with the ethanolic and n-hexane extracts (100, 200 and 400 mg/kg) exhibited significant reduction in ovalbumin induced paw edema. The preliminary phytochemical analysis revealed the presence of flavonoids, phenolic compounds, alkaloids, steroids, tannins and carbohydrates in the leaf extracts. Conclusion: From the present study, it can be concluded that the phenolic compounds and flavonoids present in M. umbellata leaves may contribute to the inhibitory effect on antigen induced mast cell degranulation and active paw anaphylaxis in Wistar rats.

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    Author(s) Name:

    N M Krishnakumar*, P G Latha, S R Suja

    Affiliation(s) Name:

    Division of Ethnomedicine and Ethnopharmacology, Jawaharlal Nehru Tropical Botanic Garden
    and Research Institute (JNTBGRI), Palode, Thiruvananthapuram-695 562, Kerala, India

    *Corresponding author.
    N M Krishnakumar
    Senior Research Fellow
    Division of Ethnomedicine and Ethnopharmacology
    Jawaharlal Nehru Tropical Botanic Garden and
    Research Institute (JNTBGRI),
    Palode, Thiruvananthapuram-695 562, Kerala, India

    Received on:14-01-2017; Revised on: 26-02-2017; Accepted on: 25-03-2017

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    Author:

    N M Krishnakumar*, P G Latha, S R Suja

    Title:Effect of Morinda umbellata Linn. (Rubiaceae) Leaf Extracts on Antigen Induced Mast Cell Degranulation and Active Paw Anaphylaxis in Wistar rats
    Journal:Journal of Pharmacy Research
    Vol(issue):11 (March)
    Year:2017
    Page No: (228-234)
  • Experimental Methods Keywords

    Methodology:Mast cell degranulation, Active paw anaphylaxis
    Research Materials:Morinda umbellata

Keywords

Mast cell degranulation Active paw anaphylaxis antigen Morinda umbellata

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