Journal: Journal of Pharmacy Research

Article Id: JPRS-P'Col-00001808
Title: In vitro investigation of some alternative therapeutic agents for antiurolithiatic activity
Category: Pharmacology
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    Background: Atherogenic hyperlipidemia, characterized by abnormal elevation in the circulatory lipoprotein levels, especially low density lipoproteins, continues to be the primary etiologic factor implicated in occlusive vascular diseases. Objectives: In the present investigation, lipid lowering effect of Calotropis procera root bark methanol extract (CPRME) and its different solvent fractions viz n-hexane, dichloromethane, ethyl acetate, methanol and aqueous fraction was studied.  Methods: Hyperlipidemia was induced by single intraperitoneal injection of Poloxamer-407 at a dose of 500 mg/kg body weight in male rats. Drug treatments were done by oral gavage at 24 h and 1 h prior to i.p. injection of P-407 and blood samples were collected at 3, 6 and 24 h intervals. The serum total cholesterol (TC), triacylglycerides (TG), high-density lipoprotein cholesterol (HDL-C) were measured while very low-density lipoprotein cholesterol (VLDL-C) and low-density lipoprotein cholesterol (LDL-C) were calculated by Friedwald formula and modified Friedwald formula respectively.  Results: From the present study, it was observed that methanolic CPRME and its ethyl acetate fraction have shown significant reduction in serum cholesterol and lipoprotein levels in P-407 induced hyperlipidemia when compared with vehicle treated positive control group. The efficacy of ethyl acetate fraction was found to be comparable to that of standard drugs- atorvastatin and fenofibrate. Conclusion: It is concluded that CPRME and its ethyl acetate fraction have shown remarkable ability to reduce serum lipids and observed effect could be attributed to the presence of vast array of phytochemicals, notably, β-sitosterol, lupeol, α- and β- amyrin in the root bark.

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    Author(s) Name:

    Ashishkumar Kyada*, Nihalahemad Mansuri1, Parula Patel

    Affiliation(s) Name:

    Department of Pharmacology, S. J. Thakkar Pharmacy College, Gujarat, India

    *Corresponding author: Ashishkumar Kyada, Department of Pharmacology, S. J. Thakkar Pharmacy College, Gujarat, India.

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    Author:

    Ashishkumar Kyada*, Nihalahemad Mansuri1, Parula Patel

    Title:In vitro investigation of some alternative therapeutic agents for antiurolithiatic activity
    Journal:Journal of Pharmacy Research
    Vol(issue):11(8)
    Year:2017
    Page No: (955-961)
  • Experimental Methods Keywords

    Methodology:Urolithiasis,nucleation assay, aggregation assay, extracted kidney stone weight reduction assay
    Research Materials:Garcinia cambogia

Keywords

Calcium oxalate Garcinia cambogia Lawsone Magnesium lactate gluconate Urolithiasis

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