Journal: Journal of Pharmacy Research

Article Id: JPRS-PCS-00001817
Title: Selective and sensitive reverse phase high-performance liquid chromatography method for the simultaneous estimation of mebeverine and chlordiazepoxide in capsule dosage form
Category: Pharmaceutics
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    Purpose: To develop a simple, linear and rapid RP-HPLC method for simultaneous determination of mebeverine and chlordiazepoxide in capsule dosage form and validate as per ICH guidelines. Methods: Separation was achieved on a Agilent C18 (250x4.6mm, 5µ particle size) column using a mobile phase consisting of methanol : TEA buffer pH 7.0 (40 : 60) (v/v) in an isocratic mode at a flow rate of 1 mL/min. The pH of the mobile phase was adjusted to 7.0 with orthophosphoric acid and UV detection was set at 262 nm. Results: The developed method resulted in mebeverine eluting at 3.4 min and chlordiazepoxide at 7.6 min. mebeverine exhibited linearity in the range 27-216μg/ml, while chlordiazepoxide exhibited linearity in the range 1.8-7.4μg/ml. Percentage Mean recoveries were found to be 100.1 and 100.3 for Mebeverine and chlordiazepoxide respectively. The limit of detection (LOD) for Mebeverine and Chlordiazepoxide were found to be 2.2μg/ml and 0.01μg/ml respectively, while limit of quantitation (LOQ) for mebeverine and mhlordiazepoxide were found to be 6.5μg/ml and 0.03μg/ml respectively. Conclusion: A simple, linear and rapid RP-HPLC method was developed for simultaneous determination of mebeverine and mhlordiazepoxide in capsules dosage form and validated as per ICH guidelines. Hence it can be used for the routine analysis of mebeverine and chlordiazepoxide in capusles in various pharmaceutical industries.

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    Author(s) Name:

    M. Madhuri1, K. E. V. Nagoji1, G. Sunitha1, T. Kiran Meena1, S. K. Sahoo2*

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    1Department of Pharmaceutical Analysis, Sri Venkateswara College of Pharmacy, Etcherla, Srikakulam, Andhra Pradesh,India,

    2Department of Pharmaceutical Chemistry, Sri Sivani College of Pharmacy, Etcherla, Srikakulam, Andhra Pradesh,India

    *Corresponding author: S. K. Sahoo, Department of Pharmaceutical Chemistry, Sri Sivani College of Pharmacy, Etcherla, Srikakulam – 532 402, Andhra Pradesh, India

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    Author:

    M. Madhuri1, K. E. V. Nagoji1, G. Sunitha1, T. Kiran Meena1, S. K. Sahoo2*

    Title:Selective and sensitive reverse phase high-performance liquid chromatography method for the simultaneous estimation of mebeverine and chlordiazepoxide in capsule dosage form
    Journal:Journal of Pharmacy Research
    Vol(issue):11(9)
    Year:2017
    Page No: (1078-1085)
  • Experimental Methods Keywords

    Methodology:Reverse phase high-performance liquid chromatography method
    Research Materials: Chlordiazepoxide, Mebeverine

Keywords

Chlordiazepoxide Mebeverine Reverse phase high-performance liquid chromatography method

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