Journal: Journal of Pharmacy Research

Article Id: JPRS-BMB-00001818
Title: In vitro and in vivo screening of antivenom activities of Vipera russelli venom by Azima tetracantha ethyl acetate extract
Category: Biochemistry and Molecular Biology
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    Aim and Objective: Azima tetracantha Lam. (Salvadoraceae) possesses pharmacologically active phytochemicals. It has antidiarrheal and antibacterial properties and is used to treat toothache and snakebites. This study evaluates the neutralizing potential of A. tetracantha on Vipera russelli venom. Materials and Methods: The ethyl acetate leaf extract (50-1500 µg/mL) was subjected to in vitro enzyme inhibition of V. russelli venom. The neutralization of procoagulant, fibrinogenolytic, hemolysis of human red blood corpuscles (HRBC), and indirect hemolytic activities was assayed by standard methods. Neutralization of lethality, edematous, hemorrhagic, and myotoxic properties of venom were analyzed in vivo using Swiss Albino mice. Lethality, hemorrhagic properties, and their neutralization were studied using chick embryos as well. Results: The study showed that the ethyl acetate extract was able to neutralize enzyme activities of venom with varying inhibitory concentration 50% (IC50) values (57.7-1086.9 μg/mL). The fibrinogenolytic and indirect hemolytic activity was reduced in 1:10 and 1:25 concentration of the extract, respectively, and in vitro hemolysis of HRBC was reduced at 60 μg/mL concentration. The neutralization of lethality (3 × lethal dose [LD50]) in mice was observed at 1:10 ratio of the extract (w/w). The hemorrhagic spot was reduced at 1:20 concentration of extract. The lactate dehydrogenase enzyme levels were reduced in myotoxicity. There was no profound effect on procoagulant and edematous activity. In chick embryos, 10 μg/μL extract concentration neutralized lethality (3 × LD50) and hemorrhagic effect of venom. Conclusion: The in vitro and in vivo studies reveals the neutralizing potential of the ethyl acetate plant extract against toxic effects of the venom.

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    Author(s) Name:

    Bhavya Janardhan1 , Vineetha M. Shrikanth1 , Bhadrapura Lakkappa Dhananjaya2 , S. A. Gautham3 , Sunil S. More3 *, M. Uday4

    Affiliation(s) Name:

    1Department of Biochemistry, Centre for Postgraduate Studies, Jain University, Bengaluru, Karnataka, India,

    2Department of Toxinology/Toxicology and Drug Discovery, Centre for Emerging Technologies, Jain Global Campus, Jain University, Kanakapura, Ramanagara - 562 112, Karnataka, India,

    3School of Basic and Applied Sciences, Dayananda Sagar University, Bengaluru, Karnataka, India,

    4Department of Biotechnology, K.L.E Technological University, Hubli, Karnataka, India

    *Corresponding author: Dr. Sunil S. More, School of Basic and Applied Sciences, Dayananda Sagar University, Bengaluru, Karnataka, India

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    Author:

    Bhavya Janardhan1 , Vineetha M. Shrikanth1 , Bhadrapura Lakkappa Dhananjaya2 , S. A. Gautham3 , Sunil S. More3 *, M. Uday4

    Title:In vitro and in vivo screening of antivenom activities of Vipera russelli venom by Azima tetracantha ethyl acetate extract
    Journal:Journal of Pharmacy Research
    Vol(issue):11(9)
    Year:2017
    Page No: (1095-1104)
  • Experimental Methods Keywords

    Methodology: The neutralization of procoagulant, fibrinogenolytic, hemolysis of human red blood corpuscles (HRBC), and indirect hemolytic activities was assayed by standard methods.
    Research Materials:: Azima tetracantha Lam. (Salvadoraceae)

Keywords

Chick embryo Hemor rhage In vivo Phospholipase A2

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