Journal: Drug Invention Today

Article Id: JPRS-PS-00001828
Title: Infusion of adrenomedullin22-52 antagonist causes uteroimplantation growth restriction during early gestation in rats
Category: Pharmacological Screening
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    Background: The present study exploited whether antagonism of endogenous Adrenomedullin (ADM) in rats during early gestation period resulted in diminished uterus and implantation growth and explored whether this causes through induction of apoptosis. Rats on gestational day 2 were implanted subcutaneously with osmotic (ALZET) minipumps delivering 125 and 250 µg/rat/day/of ADM22-52 and were killed on gestational day 9. On gestational day 9, both in the control group rats and ADM antagonist-treated rats, 5-bromo 2´deoxyuridine labeling dye is injected in the tails of rats with a concentration of 1 ml/100 g body weight and then sacrificed the animal. We have demonstrated that the uterus growth and implantation development are seriously compromised by this modest decrease in expression and the implanted sites were shown prominently by blue-/purple-colored spots both in control and ADM-treated groups. Results: Apoptotic variations were more evident in stromal trophoblastic cells in the uteroimplantation sites of ADM22-52-treated rats when compared with vehicle control rats. Immunoreactivity to active caspase-3, Bax, Bcl2 protein was abundant in the uteroimplantation regions of the ADM22-52-treated group. Conclusion: These findings, however, show that antagonism of ADM in rats during early pregnancy caused uteroimplantation growth restriction and increased fetal resorption through the activation of mitochondrial apoptotic pathways.

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    Author(s) Name:

    K. R. Padma, G. Lakshmi Deepika, P. Josthna

    Affiliation(s) Name:

    Department of Biotechnology, Sri Padmavati Mahila Visvavidyalayam (Women’s University), Tirupati, Andhra Pradesh, India

    *Corresponding author: K. R. Padma, Department of Biotechnology, Sri Padmavati Mahila Visvavidyalayam (Women’sUniversity), Tirupati - 517 502, Andhra Pradesh, India.

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    Author:

    K. R. Padma, G. Lakshmi Deepika, P. Josthna

    Title:Infusion of adrenomedullin22-52 antagonist causes uteroimplantation growth restriction during early gestation in rats
    Journal:Drug Invention Today
    Vol(issue):9(2)
    Year:2017
    Page No: (28-34)
  • Experimental Methods Keywords

    Methodology:Infusion of Osmotic (ALZET) Pumps,Collection of Uteroimplantation Regions from Early Pregnant Rats,BrDU Incorporation Assay,Histopathological Examination
    Research Materials: Adrenomedullin (ADM)

Keywords

Adrenomedullin Preeclampsia Uteroimplantation

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