Journal: Journal of Pharmacy Research

Article Id: JPRS-P'Col-00001933
Title: Correction of retinal ischemia/reperfusion by 3-(1H-benzimidazol-2-il)-1,2,2-trimethyl cyclopentanecarboxylic acid in experiment
Category: Pharmacology
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    Introduction: Studying the way of how to improve tissue tolerance to ischemia is an actual problem of pharmacology. Up to now, the treatment of ischemic retinal conditions was done by use of angioprotectors, antioxidants, fibrinolytics, anticoagulants, and others. Due to the instability and short-term effects after using these drugs and physiotherapy treatments is necessary to seek out a more effective way to improve blood circulation and increase resistance to ischemic retinal tissue. Research tasks: The aim of this study is to increase the effectiveness of pharmacological correction of retinal ischemia-reperfusion using agonist of imidazoline receptors Type I, II, 3-(1H-benzimidazol-2-il)-1,2,2-trimethyl cyclopentanecarboxylic acid. Methods: To investigate the fundus of experimental animals, a direct ophthalmoscopy was
    used. To zoom a lens, Osher MaxField 78D model OI-78M has been used. Electroretinography (ERG) was performed at once after the ophthalmoscopy. To assess the degree of functional damage to the retina, we evaluated the ratio of amplitudes of a- and b-waves - the coefficient b/a. For all data, the descriptive statistics were used, and data are checked for normal distribution. Distribution type was determined using the criterion of Shapiro–Wilk. Between groups, differences were analyzed by parametric (t-Student criterion) or non-parametric (Mann–Whitney test) methods, depending on the type of distribution. Differences were determined at 0.05 significant level. Results: The protective effect of agonist of imidazoline receptors Type I, II, 3-(1H-benzimidazol-2-il)-1,2,2-trimethyl cyclopentanecarboxylic acid, in doses 10 mg/kg and 50 mg/kg on the retinal ischemia-reperfusion model on Wistar rats was studied. In the experiment, it was found that the 3-(1H-benzimidazol-2-il)-1,2,2-trimethyl cyclopentanecarboxylic acid in a dose 50 mg/kg prevents the development of neuronal damage in the retina caused by intraocular pressure increase to 110 mmHg within 30 min by applying the mechanical pressure to the anterior chamber of the eye to a greater extent than in a dose 10 mg/kg. The detected protective effects were confirmed by the results of ophthalmoscopy and ERG after 72 h of reperfusion. Conclusion: Results of ocular fundus studies revealed the most pronounced protective effects of 3-(1H-benzimidazol-2-il)-1,2,2-trimethyl cyclopentanecarboxylic acid in a dose 50 mg/kg on the model of retinal ischemia-reperfusion in Wistar rats, which is reflected in the restoration of the optic disc. Correction of retinal ischemia-reperfusion by 3-(1H-benzimidazol-2-il)-1,2,2-trimethyl cyclopentanecarboxylic acid in a dose 50 mg/kg leads to higher values of the coefficient b/a of ERG after 72 h of reperfusion compared to the group with pathology correction by the same drug in a dose 10 mg/kg, which indicates the restoration of the electrophysiological state of the retina.

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    Author(s) Name:

    Anna A. Peresypkina1*, Elena A. Levkova1, Vladimir I. Yakushev1, Natalia D. Bunyatyan2, Pavel A. Galenko-Yaroshevsky3, Mikhail I. Churnosov1

    Affiliation(s) Name:

    1Department of Pharmacology, Belgorod State University, Belgorod, Russia,
    2FSBI “Scientific Center for Expertise of Medical Applications” of the Ministry of Health of the Russian Federation, 8(2), Petrovsky boulevard, Moscow, Russia,
    3Depatment of Pharmacology, Kuban State Medical University, Krasnodar, Russia

    *Corresponding author: Anna A. Peresypkina, Department of Pharmacology, Belgorod State National Research University 85, Pobedy Street, Belgorod - 308015, Russia.

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    Author:

    Anna A. Peresypkina1*, Elena A. Levkova1, Vladimir I. Yakushev1, Natalia D. Bunyatyan2, Pavel A. Galenko-Yaroshevsky3, Mikhail I. Churnosov1

    Title:Correction of retinal ischemia/reperfusion by 3-(1H-benzimidazol-2-il)-1,2,2-trimethyl cyclopentanecarboxylic acid in experiment
    Journal:Journal of Pharmacy Research
    Vol(issue):11 (December )
    Year:2017
    Page No: (1552-1555)
  • Experimental Methods Keywords

    Methodology: Electroretinography, Ophthalmoscopy, Retinal ischemia-reperfusion
    Research Materials: 3-(1H-benzimidazol-2-il)-1,2,2-trimethyl cyclopentanecarboxylic acid

Keywords

3-(1H-benzimidazol-2-il)-1 2 2-trimethyl cyclopentanecarboxylic acid Electroretinography Ophthalmoscopy Retinal ischemia-reperfusion

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