Journal: Drug Invention Today

Article Id: JPRS-PS-00001937
Title: Morphological characteristics of the rats brain under hypergravity
Category: Pharmacological Screening
Section: Research Article
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    Aim: The objective of the research was to study the morphological changes in the most sensitive areas of the brain under hypergravity, using standard general histological and histochemical, as well as immunohistochemical methods with antibodies to glial fibrillary acid protein, neurofilament protein, Ki67 proliferation markers, and p53 apoptosis. Materials and Methods: The experiment was performed on 40 white Wistar rats divided into 4 groups: 1st - control, 2nd - rats exposed to hypergravity for 8 min at a centrifuge rotation rate of 5,000 rpm, 3rd - rats exposed to hypergravity 3 times during the 1st day for 5 min and at a rotation rate of 4,000 rpm, and 4th - rats exposed to hypergravity 3 times for 5 min and a rotation speed of 4,000 rpm for 3 days.After the end of the experiment, morphological changes in the brain were examined. Results: It was established that the animals of the first
    group died from compression and dislocation of the brain resulted from overloads. Rats from the second group had clinical signs, such as weakness, one- and two-sided paresis, which completely passed within an hour. The histological picture of the brain had no specific features. In the third and fourth groups, the clinical symptoms were more pronounced, remaining until the end of the 1st day. Morphological picture of the immunohistochemical examination had no features but showed severe microcirculation disorders when stained with hematoxylin and eosin. Conclusion: Thus, the hypergravitational model of cerebral ischemia allows
    obtaining circulatory hypoxia of the brain, which is confirmed by clinical data and general histological methods.

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    Author(s) Name:

    Aleksei V. Tverskoi*, Vitaliy N. Morozov, Anastasia A. Petrenko, Tatyana S. Mukhina, Aleksandr S.Parichuk

    Affiliation(s) Name:

    Department of Human Anatomy and Histology, Belgorod State University 308015, Belgorod, Russia

    *Corresponding author: Aleksei V. Tverskoi, Department of Human Anatomy and Histology, Belgorod State University308015, Belgorod, Pobeda Street 85, Russia

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    Author:

    Aleksei V. Tverskoi*, Vitaliy N. Morozov, Anastasia A. Petrenko, Tatyana S. Mukhina, Aleksandr S.Parichuk

    Title:Morphological characteristics of the rats brain under hypergravity
    Journal:Drug Invention Today
    Vol(issue):9 (December)
    Year:2017
    Page No: (30-32)
  • Experimental Methods Keywords

    Methodology:Cerebral ischemia, Hippocampus, Hypergravity, Morphological changes
    Research Materials:Rats brain

Keywords

Cerebral ischemia Hippocampus Hypergravity Morphological changes Rats

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