Journal: Drug Invention Today

Article Id: JPRS-D(DD)-00001967
Title: Development of a technique for assessing the quality of propolis by the content of caffeic acid phenethyl ester by high-performance liquid chromatography with a diode- matrix detector
Category: Drug (Discovery/Development)
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    Propolis is characterized by a wide range of pharmacological properties, such as antioxidant, anti-inflammatory, immunostimulating, hepatoprotective, and antidiabetic, as well as cardioprotective. A number of listed pharmacological properties of propolis is associated primarily with of oxycinnamic acid ester, one of them - caffeic acid phenethyl ester (CAPE) - is considered the most pharmacologically significant. This paper presents the results of the development of an estimation of the quality of propolis with the use of CAPE as a marker component. Using reversed-phase high-performance liquid chromatography with diode-matrix detection, identification was carried out, and the quantitative content of this component in propolis was determined. As an optimal elution mode, a gradient regime was used, with allowed differentiating CAPE from other esters of caffeic acid. In the course of the experiment, it was found that the most suitable extractant for extracting CAPE from propolis is isopropyl alcohol. The optimal color form of propolis, containing the greatest amount of the component to be determined, is green propolis. A quantitative evaluation of the studied component was carried out using the absolute calibration method in the concentration range of the standard sample of CAPE 0.01–0.1%. In the indicated concentration range, the calibration curve was linear; the correlation coefficient was R² = 0.9998. To assess the comparability and correctness of the methodology, metrological characteristics were calculated. The error of the single determination with the relative error P, 95% was 4.76%. The quantitative content of CAPE in 6 propolis samples taken from different regions of the Russian Federation showed a significant difference in the content of this component and was in the range of 0.106– 0.515%. The results of the conducted studies indicate the possibility of assessing the quality of propolis in terms of one of its most significant components - CAPE.

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    Author(s) Name:

    Dmitriy I. Pisarev*, Oleg O. Novikov, Anastasiya Yu. Malyutina, Ekaterina V. Lupina, Elena T. Zhilyakova, Nikolay N. Boyko

    Affiliation(s) Name:

    Department of Pharmaceutical Chemistry and Pharmacognosy, Belgorod State University, Belgorod, Russia

    *Corresponding author: Dmitriy I. Pisarev, Department of Pharmaceutical Chemistry and Pharmacognosy, Belgorod State University, Belgorod, Pobedy Street, 85, 308015, Russia

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    Author:

    Dmitriy I. Pisarev*, Oleg O. Novikov, Anastasiya Yu. Malyutina, Ekaterina V. Lupina, Elena T. Zhilyakova, Nikolay N. Boyko

    Title:Development of a technique for assessing the quality of propolis by the content of caffeic acid phenethyl ester by high-performance liquid chromatography with a diode- matrix detector
    Journal:Drug Invention Today
    Vol(issue):9 (December)
    Year:2017
    Page No: (53-58)
  • Experimental Methods Keywords

    Methodology:Method of absolute graduation, Propolis, Reversed-phase high-performance liquid chromatography
    Research Materials:Caffeic acid phenethyl ether

Keywords

Caffeic acid phenethyl ether Method of absolute graduation Propolis Reversed-phase high-performance liquid chromatography

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