Journal: Journal of Pharmacy Research

Article Id: JPRS-P'Col-00001977
Title: Methods of scanning blood microscopy in oncology diagnosis of kidney pathology
Category: Pharmacology
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    Background: The purpose of this article is to reveal pathomorphological changes in erythrocytes with oncological processes in the kidneys with the help of atomic force microscopy (AFM) and scanning electron microscopy (SEM). Methods: Erythrocytes of patients with kidney pathology are the object of this research. After allocation of erythrocytes, blood samples from a vein were studied in SEMs “FE1 Quanta 200 three-dimensional” and “FE1 Quanta 600 FEG.” AFM was done in the probe laboratory Ntegra Aura (Russia). The studies were performed in contact modes of intermittent or constant profiles using commercial Si or SiN cantilevers (NSG01, NT-MDT, Russian Federation) under conditions of low atmospheric vacuum. AFM images were processed and implemented using the NOVA software (NT-MDT, Russian Federation) and image analysis (NT-MDT, Russian Federation). Results: The number of transient forms of erythrocytes is increased with aging of patients. There was a decrease of discocytes and an increase of the number of transitional, prehemolytic and degenerative cell forms with kidney cancer. When studying the appearance of erythrocytes with the help of AFM, it was established that the depth of the discocyte cavity counted by studying the cell profile was significantly changing. With the progression of the disease, the cells acquired elongated forms, as well as contacts between the cells, were more common. Conclusions: The study has found that the appearance and condition of erythrocyte membranes depend on the age of the recipient and to the greater extent on pathological processes in the kidneys (cysts and cancer) with a change of their cytoarchitecture. The greatest changes have been found in kidney cancer with distant metastases. Modified cells are functionally defective in part and cannot fulfill their most important oxygen transport function in full measure.

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    Author(s) Name:

    Tatyana V. Pavlova*, Ivan A. Pavlov, Natalia B. Pilkevich, Marina A. Chaplyginа

    Affiliation(s) Name:

    Department of Pathology, Belgorod State University, Belgorod, Russia

    *Corresponding author: Prof. Tatiana V. Pavlova, Department of Pathology, Belgorod State University, 85, Pobeda St., Belgorod, 308015, Russia.

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    Author:

    Tatyana V. Pavlova*, Ivan A. Pavlov, Natalia B. Pilkevich, Marina A. Chaplyginа

    Title:Methods of scanning blood microscopy in oncology diagnosis of kidney pathology
    Journal:Journal of Pharmacy Research
    Vol(issue):11 (December )
    Year:2017
    Page No: (1589-1593)
  • Experimental Methods Keywords

    Methodology:Atomic force microscopy,Oncodiagnosis, Scanning electron microscopy
    Research Materials:Erythrocytes

Keywords

Atomic force microscopy Erythrocytes Kidney cancer Oncodiagnosis Scanning electron microscopy

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