Journal: Journal of Pharmacy Research

Article Id: JPRS-PCS-0000199
Title: Development and Validation For The Simultaneous Estimation of Sparfloxacin and Dexamethasone by Reverse Phase High Performance Liquid Chromatographic Method
Category: Pharmaceutics
Section: Research Article
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    In the present study an RP-HPLC method was developed and validated for the simultaneous determination of Sparfloxacin and Dexamethasone. The chromatographic system was equipped with SUPELCO 516 C18 column and UV detector set at 254 nm, in conjunction with a mobile phase of 0.05 M Potassium Dihydrogen Phosphate buffer and Acetonitrile in the ratio of 50:50 (pH 4, adjusted with orthophosphoric acid) at a flow rate of 1.0 ml/min and the injection volume set at 20 µl with 10 minutes of runtime. The described method was linear over a concentration range of 5-45 µg/ml for the simultaneous estimation of Sparfloxacin and Dexamethasone with a with good linearity response of 0.9996 and 0.9979 respectively. The retention time of Sparfloxacin was 4.127 min ± 0.16 and of Dexamethasone was 5.727 min ± 0.10. The results of analysis were validated. The results of the study showed that the proposed RP-HPLC method was simple, rapid, precise and accurate, which is useful for the routine determination of Sparfloxacin and Dexamethasone in bulk drug and in pharmaceutical dosage form.

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    Author(s) Name:

    R.J. Mahida 1*, M.A.Hinge1

    Affiliation(s) Name:

    1Rofel Shri G. M. Bilakhia College of Pharmacy, Vapi, Gujarat, India.

     

    *Corresponding author.
    R.J. Mahida
    Rofel Shri G. M. Bilakhia College of Pharmacy,
    Vapi , Gujarat, India


    Received on:20-04-2014; Revised on: 05-05-2014; Accepted on:11-05-2014

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    Author:

    R.J. Mahida 1*, M.A.Hinge1

    Title:Development and Validation For The Simultaneous Estimation of Sparfloxacin and Dexamethasone by Reverse Phase High Performance Liquid Chromatographic Method
    Journal:Journal of Pharmacy Research
    Vol(issue):8 (May)
    Year:2014
    Page No: (650-654)
  • Experimental Methods Keywords

    Methodology:RP-HPLC method
    Research Materials:Sparfloxacin, Dexamethasone

Keywords

Sparfloxacin Dexamethasone RP-HPLC and Acetonitrile Simultaneous estimation.

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