Journal: Journal of Pharmacy Research

Article Id: JPRS-PB-00001991
Title: Vitamin D status influences mitochondrial metabolic activity and hyperglycemic condition of skeletal muscle cells
Category: Pharmaceutical Biotechnology
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    Background: In the recent past, vitamin D deficiency has been found to be associated with diabetes. As per different studies, an adequate level of vitamin D is important for various cellular activities such as insulin secretion, insulin signalling and glucose uptake by target cells. In addition to vitamin D deficiency, the mitochondrial defect is also known to be an important factor causing hyperglycemia. Though vitamin D deficiency and mitochondrial defects have the same consequence on glucose metabolism, however, there are very few reports available which suggest that vitamin D also affect the mitochondrial defects. Objective: Our current study aims to understand the possible mechanism and link between vitamin D deficiency leading to mitochondrial defects and further causing the hyperglycemia. Material and Methods: The rat skeletal muscle cells (L6) were grown in normal (8mM) and high (25mM) glucose concentration. The cells were further supplemented with MTT optimized dose of vitamin D (10-9 M) and grown at different till different time intervals of 24, 48 and 72 hrs. Results: As a result we have demonstrated that vitamin D supplementation to L6 cells have shown the improved calcium uptake which is a preliminary parameter for vitamin D activity. Ca++ being an important cofactor of different enzymes of Kreb’s cycle, also showed the improved mitochondrial enzyme activity in correlation with increased calcium uptake. The calcium uptake and NADH oxido-reductase activity was also in coordination with improved mitochondrial membrane potential, improved catalase activity and increased glucose uptake by these cells and different time intervals, when supplementated with vitamin D. Conclusion: Thus as per or study we conclude that vitamin D deficiency initially affect the mitochondrial metabolism which ultimately affect the glucose uptake by target cells and causes hyperglycemia. Thus vitamin D supplementation can be suggested as a part of therapy to the patients suffering from diabetes.

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    Author(s) Name:

    Nancy Taneja, Shalini Mani*

    Affiliation(s) Name:

    Department of Biotechnology, Jaypee Institute of Information Technology, Noida, Uttar Pradesh, India

    *Corresponding author: Shalini Mani, Senior Assistant Professor, Department of Biotechnology, Jaypee Institute of Information Technology, Noida, Uttar Pradesh, India.

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    Author:

    Nancy Taneja, Shalini Mani*

    Title:Vitamin D status influences mitochondrial metabolic activity and hyperglycemic condition of skeletal muscle cells
    Journal:Journal of Pharmacy Research
    Vol(issue):12 (February )
    Year:2018
    Page No: (220-226)
  • Experimental Methods Keywords

    Methodology:rat skeletal muscle cells (L6),mitochondrial metabolic activity, hyperglycemic condition
    Research Materials:Vitamin D

Keywords

Catalase Glucose Mitochondria Vitamin D

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