Journal: Drug Invention Today

Article Id: JPRS-MSNS-00002015
Title: Inhibitory spectra of isolated tannin-rich fraction and assessment of compounds using chromatographic techniques
Category: Microbiological Study (on Natural/ Synthetic drug)
Section: Research Article
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    Introduction: Tannins, secondary metabolites of plants, are mostly water-soluble phenolic compounds exhibit numerous biological and antibacterial-promoting and antioxidant effects. Objective: The aim of the study is to characterize the various classes of tannins and to screen their biological activity. Materials and Methods: Isolation of tannin-rich fraction was done by means of column chromatography and assessment of compounds was done using bioassay techniques. Characterization of compounds was analyzed by means of ultraviolet (UV)-visible, Fourier-transform infrared spectroscopy (FTIR), and reverse phase-high performance liquid chromatography (RP-HPLC). Results: Antimicrobial screening reveals that Syzygium aromaticum fraction 2 (21.6 mm) and 3 (18.3 mm) exhibit broad spectrum of activity followed by Candida glabrata (17.5 mm). Screening of tannin-rich fraction indicates that Escherichia coli, Proteus mirabilis, and C. glabrata remained sensitive toward the fraction, whereas Salmonella typhi, Aspergillus Parasiticus, and Fusarium solani remained resistant toward the fraction possessing least inhibition zone. UV absorption spectrum reveals the presence of condensed tannin, gallotannin, and ellagic acid (EA). FTIR spectrum denotes the presence of fingerprint region of hydrolysable tannin. RPHPLC analysis indicates the presence of gallic acid, EA, epicatechin, and catechin. Conclusion: The plant materials such as S. aromaticum and Acorus calamus indicate the presence of hydrolysable, condensed, and gallotannins.

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    Author(s) Name:

    Rajeswari Anburaj1 *, Vinoth jothiprakasam2

    Affiliation(s) Name:

    1 Department of Microbiology, MIET Arts and Science College, Affiliated to Bharathidasan University, Tiruchirapalli, Tamil Nadu, India,

    2 CAS in Marine Biology, Annamalai University, Parangipettai, Tamil Nadu, India

    *Corresponding author: A. Rajeswari, Assistant Professor, MIET Arts and Science College, Department of Microbiology, Bharathidasan University, Tiruchirapalli - 620 024, Tamil Nadu, India

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    Rajeswari Anburaj1 *, Vinoth jothiprakasam2

    Title:Inhibitory spectra of isolated tannin-rich fraction and assessment of compounds using chromatographic techniques
    Journal:Drug Invention Today
    Vol(issue):9 (December)
    Page No: (74-87)
  • Experimental Methods Keywords

    Methodology: Antimicrobial screening, Fourier-transform infrared spectroscopy,Reverse phasehigh-performance liquid chromatography
    Research Materials:Tannin-rich fraction ,Phenolic compounds


Antimicrobial screening Fourier-transform infrared spectroscopy Phenolic compounds Reverse phasehigh-performance liquid chromatography Tannin

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