Journal: Drug Invention Today
Title: Kras, epidermal growth factor receptor, and p53, but not Nras or Braf, biomarkers are frequently altered in pancreatic adenocarcinoma and precursor lesions
Category: Pharmacological Screening
Section: Research Article
- Abstract
- Audio Abstract
- Authors
- Pdf File
- Citation
- My Reference
- Methodology
-
Abstract
Aim: Pancreatic cancer is estimated to be the 12th most common cancer in men and the 11 most common in women worldwide. Materials and Method: As other cancers, pancreatic ductal adenocarcinoma (PDAC) accumulates genetic alterations in oncogenes and suppressor genes. In the present study, we analyzed five biomarkers status: Epidermal growth factor receptor (EGFR) and p53 using immunohistochemical labeling in 39 PDAC and Kras, Nras, and Braf, using pyrosequencing in 25 normal pancreatic tissues, 25 pancreatic intraductal neoplasia (PanIN), and 39 PDAC. Result and Discussion: Abnormal immunolabeling of p53 and EGFR was detected in 97.4% and 71.8%, respectively. There was a statistically significant progressive increment (P < 0.001) in the percentage of mutated cases through normal pancreas (8%), PanIN-1A (28.6%), PanIN-1B (33.3%), PanIN-2 (60%), and PanIN-3 (85.7%) to PDAC (94.8%). These mutations were arising in codons 12 and 61 of Kras and in codon 600 of Braf. Most frequent mutations were G12D (46%), G12A (23%), and G12V (18%) of Kras. No mutations were found in codons 13, 59, 117, and 146 of Kras or in codons 12, 13, 59, 61, 117, and 146 of Nras. Conclusion: We confirm that EGFR, Kras, and p53 are the most frequent altered biomarkers in PDAC with difference in predominated Kras mutations subtypes.
-
Abstract Audio
No Audio file found
About the authors and Affiliations
Author(s) Name: Marwa Ferchichi 1,2 , Raja Jouini 2 *, Imen Ayari 1,2 , Aschraf Chadli-Debbiche2 , Ehsen Ben Brahim2
Affiliation(s) Name: 1 Department of Physique, University of Sciences, Farhat Hached Campus, Tunis El Manar, Tunisia,
2Department of Pathology, Habib Thameur Hospital, Tunis, Tunisia
*Corresponding Author: Raja Jouini, University of Sciences, Farhat Hached Campus, Tunis El Manar, Tunisia.
-
View Article File in pdf format.
Article File
-
View Article Citation Here.
0 View More -
How to Cite my Article.
Author: Marwa Ferchichi 1,2 , Raja Jouini 2 *, Imen Ayari 1,2 , Aschraf Chadli-Debbiche2 , Ehsen Ben Brahim2
Title: Kras, epidermal growth factor receptor, and p53, but not Nras or Braf, biomarkers are frequently altered in pancreatic adenocarcinoma and precursor lesions Journal: Drug Invention Today Vol(issue): 9 (December) Year: 2017 Page No: (88-96) -
Experimental Methods Keywords
Methodology: Molecular Analysis, Mutations Analysis, Statistical Analysis Research Materials: Pancreatic cancer
Keywords
Our Services
- Evaluation of Cardioprotective Role of Vinpocetine in Isoproterenol-induced Myocardial Infarction in Rats
- Solubility and Dissolution Enhancement: An overview
- In vitro antioxidant and ptp inhibitory activity of methanolic extract of Anogeissus acuminata leaf and bark.
- A review on Calotropis procera Linn and its Ethnobotany, Phytochemical, Pharmacological profile
- Antimicrobial activity and phytochemical screening of five wild plants against Escherichia coli, Bacillus subtilis and Staphylococcus aureus
- A review on quality by design approach for analytical method development
- Development and Validation of Stability Indicating UV Spectrophotometric Method for the Estimation of Sitagliptin Phosphate in Bulk and Tablet Dosage Form
- Evaluation of Phenolic Content and Antioxidant Capacity of leaf extract from Phoenix Dactylifera L obtained by different pH of aqueous extraction
- Benzoic acid, one of the major components of aqueous bark extract of Terminalia arjuna protects against Copper-Ascorbate induced oxidative stress in human placental mitochondria through antioxidant m
- Zinc-silibinin complex ameliorates oxidative stress in high fat fed low dose STZ induced type 2 diabetes in rats
- Biological synthesis of silver nanoparticles using aqueous leaf extract of Capparis decidua (FORSK.) EDGEW: A better alternative
- Formulation and evaluation of topical gel of diclofenac sodium using different polymers
- Study of health related quality of life in rheumatoid arthritis patients
- Prescribing Patterns of antihypertensive drugs in a South Indian tertiary care hospital
- Antidiabetic activity of insulin plant (Costus igneus) leaf extract in diabetic rats
- In vitro antioxidant, anti-inflammatory and anticholinesterase activities of Rumex vesicarius L
- Antimicrobial activity of seaweeds Gracillaria, Padina and Sargassum sps. on clinical and phytopathogensSubba Rangaiah G, Lakshmi P* and Manjula E
- Gastroprotective effect of Fenugreek 4-hydroxyisoleucine and trigonelline enriched fraction (TF4H (28%)) Sugaheal® against indomethacin induced ulcer in male wistar rats.
- In vitro anti-Candida activity of Calotropis gigantea.
- Pharmacognostic, Phytochemical & Quantitative Investigation of Saraca asoca leaves
- Anti ulcer activity of Naravelia zeylanica leaves extract
- Melatonin affords protection against myocardial ischemia-induced cerebral mitochondrial dysfunction: an in vivo study.
- Effects of Osmolytes on The Structural Stability of Bovine Trypsin: A Brief Review
- Synthesis and Biological Activity of Novel Acyl Hydrazone Derivatives of 3-(4,5-diphenyl-1,3-oxazol-2-yl) propanoic acid as Anticancer, Analgesic and Anti-inflammatory Agents
- Design and Synthesis of Novel Quinazolinone Derivatives as Broad Spectrum Anticonvulsant and Antimicrobial Agent
- In vitro Antioxidant Activity from Leaves of Oroxylum indicum (L.) Vent. -A North Indian Highly Threatened and Vulnerable Medicinal Plant
- Assessment of Pharmacognostical Characters of Leaves of Tephrosia Purpurea Linn
- Genome of Acinetobacter baumannii Carries Multi Drug Resistance Genes
- Triazole analogues as novel antioxidants: In vitro bioassay and molecular modeling studies
- Anticonvulsant And Antioxidant Actions Of Some Dibenzo-a-Pyrone Derivatives In Pentylene –Induced Kindling Model In Mice