Journal: Drug Invention Today

Article Id: JPRS-PS-00002016
Title: Kras, epidermal growth factor receptor, and p53, but not Nras or Braf, biomarkers are frequently altered in pancreatic adenocarcinoma and precursor lesions
Category: Pharmacological Screening
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  • Abstract

    Aim: Pancreatic cancer is estimated to be the 12th most common cancer in men and the 11 most common in women worldwide. Materials and Method: As other cancers, pancreatic ductal adenocarcinoma (PDAC) accumulates genetic alterations in oncogenes and suppressor genes. In the present study, we analyzed five biomarkers status: Epidermal growth factor receptor (EGFR) and p53 using immunohistochemical labeling in 39 PDAC and Kras, Nras, and Braf, using pyrosequencing in 25 normal pancreatic tissues, 25 pancreatic intraductal neoplasia (PanIN), and 39 PDAC. Result and Discussion: Abnormal immunolabeling of p53 and EGFR was detected in 97.4% and 71.8%, respectively. There was a statistically significant progressive increment (P < 0.001) in the percentage of mutated cases through normal pancreas (8%), PanIN-1A (28.6%), PanIN-1B (33.3%), PanIN-2 (60%), and PanIN-3 (85.7%) to PDAC (94.8%). These mutations were arising in codons 12 and 61 of Kras and in codon 600 of Braf. Most frequent mutations were G12D (46%), G12A (23%), and G12V (18%) of Kras. No mutations were found in codons 13, 59, 117, and 146 of Kras or in codons 12, 13, 59, 61, 117, and 146 of Nras. Conclusion: We confirm that EGFR, Kras, and p53 are the most frequent altered biomarkers in PDAC with difference in predominated Kras mutations subtypes.

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    Author(s) Name:

    Marwa Ferchichi 1,2 , Raja Jouini 2 *, Imen Ayari 1,2 , Aschraf Chadli-Debbiche2 , Ehsen Ben Brahim2

    Affiliation(s) Name:

    1 Department of Physique, University of Sciences, Farhat Hached Campus, Tunis El Manar, Tunisia,

    2Department of Pathology, Habib Thameur Hospital, Tunis, Tunisia

    *Corresponding Author: Raja Jouini, University of Sciences, Farhat Hached Campus, Tunis El Manar, Tunisia.

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    Author:

    Marwa Ferchichi 1,2 , Raja Jouini 2 *, Imen Ayari 1,2 , Aschraf Chadli-Debbiche2 , Ehsen Ben Brahim2

    Title:Kras, epidermal growth factor receptor, and p53, but not Nras or Braf, biomarkers are frequently altered in pancreatic adenocarcinoma and precursor lesions
    Journal:Drug Invention Today
    Vol(issue):9 (December)
    Year:2017
    Page No: (88-96)
  • Experimental Methods Keywords

    Methodology:Molecular Analysis, Mutations Analysis, Statistical Analysis
    Research Materials:Pancreatic cancer

Keywords

Epidermal growth factor receptor Kras Pancreatic intraductal neoplasia Pancreatic ductal adenocarcinoma p53

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