Journal: Journal of Pharmacy Research

Article Id: JPRS-MB-00002051
Title: β (1-3) glucan production from Agrobacterium fabrum
Category: Microbiology
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    Aim: The present study was focused on the production of β (1-3)-glucan from Agrobacterium sp. Among the 50 isolates, 1 was selected for the production after the screening. The production media were optimized with different carbon and nitrogen sources. Fourier-transform infrared (FT-IR) was used for the characterization of the product. Materials and Methods: Root nodule samples were obtained from Coimbatore and Palakkad for the isolation of Agrobacterium sp. Aniline blue method was used as the screening test for the production of β (1-3) glucan. The positive isolate was identified by 16S rRNA sequencing. The minimal salt medium with carbon and nitrogen source was used as the production medium. The crude β (1-3) glucan was obtained by sodium hydroxide and acetic acid precipitation method. Characterization was done by FT-IR analysis. Result and Discussion: The organism was confirmed as Agrobacterium fabrum by 16S rRNA. The GenBank accession number for isolate is MF521602. 0.12 g/100 mL of β (1-3) glucan was recovered from the medium. The transmission spectrum of FT-IR indicated the presence of β (1-3) glucan. Conclusion: The conducted study showed that sucrose and ammonium chloride were the best sources for the production of β (1-3) glucan.

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    Author(s) Name:

    V. Muthu Laxmi*, D. Latha

    Affiliation(s) Name:

    Division of Microbiology, School of Biological Sciences, CMS College of Science and Commerce, Coimbatore, Tamil Nadu, India

    *Corresponding Author: V. Muthu Laxmi, Division of Microbiology, School of Biological Sciences, CMS College of Science and Commerce, Coimbatore, Tamil Nadu, India.

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    Author:

    V. Muthu Laxmi*, D. Latha

    Title:β (1-3) glucan production from Agrobacterium fabrum
    Journal:Journal of Pharmacy Research
    Vol(issue):12(4)
    Year:2018
    Page No: (484-488)
  • Experimental Methods Keywords

    Methodology:16S rRNA sequencing, Acetic acid precipitation, GenBank accession, FT-IR analysis
    Research Materials: Agrobacterium fabrum

Keywords

16S rRNA Acetic acid precipitation Agrobacterium fabrum GenBank accession Sodium hydroxide

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